Description

Rec A Protein for Molecular Biology is a highly purified, recombinant enzyme essential for in vitro DNA recombination and repair studies. This product is critical for researchers requiring a reliable and active protein to facilitate homologous pairing and strand exchange reactions with precision. It is primarily used by molecular biologists, geneticists, and R&D teams in biotechnology and pharmaceutical sectors for advanced genetic engineering, mutation studies, and functional genomics.

Application

• In vitro Homologous Recombination Assays: Essential for studying the molecular mechanisms of DNA repair and genetic exchange.
• Strand Exchange and Pairing Reactions: Used to catalyze the pairing of homologous DNA molecules and the exchange of DNA strands.
• Genetic Engineering and Synthetic Biology: A key tool for constructing recombinant DNA molecules and manipulating genetic circuits.
• DNA Damage Response Research: Facilitates the study of cellular responses to DNA damage and repair pathway analysis.
• Biochemical Reaction Standardization: Serves as a critical component in standardizing and optimizing enzymatic recombination protocols.
• High-Throughput Screening (HTS): Used in drug discovery platforms targeting DNA repair enzymes for therapeutic development.

Basic Information

Product Name	Rec A Protein for Molecular Biology
CAS No.	155979-15-0
Molecular Formula	Contact for details
Molecular Weight	~38 kDa (monomer)
Synonyms	Recombinase A Protein; RecA; ATP-dependent recombinase A; DNA strand-exchange protein; RecA protease; RecA gene product; Escherichia coli RecA protein; EC 3.6.4.-
EINECS	Contact for details

Quality Control

Our Rec A Protein for Molecular Biology is manufactured under stringent conditions to ensure high activity, purity, and lot-to-lot consistency. Each batch is subjected to rigorous quality testing, including activity assays, purity analysis, and absence of contaminating nucleases. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, buffer composition, and storage conditions is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. For long-term storage, aliquot to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 95%
Specific Activity	≥ 5,000 units/mg protein
Protein Concentration	2-5 mg/mL
Buffer	20 mM Tris-HCl, pH 7.5, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
DNase/RNase Activity	Not detected
Protease Activity	Not detected
Endotoxin Level	< 1.0 EU/µg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.