Description

L-Pyroglutamyl-L-Arginyl-L-Threonyl-L-Lysyl-L-Arginine 4-Methylcoumaryl-7-Amide is a synthetic fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and quality control laboratories requiring precise and reliable detection of specific protease activity. It is primarily utilized in the fields of biochemistry, pharmaceutical research, and diagnostic development for studying enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: As a selective fluorogenic substrate for the detection and quantification of specific trypsin-like serine proteases.
• Biochemical Research: Used in kinetic studies to determine enzyme parameters such as Km and Vmax, and for characterizing protease inhibitors.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and validate novel therapeutic protease inhibitors.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for clinical and research use, detecting specific enzymatic markers.
• Cell Biology Studies: Applied in assays to monitor protease activity within cellular systems and study proteolytic signaling pathways.
• Quality Control (QC) Testing: Used in biopharmaceutical QC labs to monitor the activity and consistency of enzyme-based products during manufacturing.

Basic Information

Product Name	L-Pyroglutamyl-L-Arginyl-L-Threonyl-L-Lysyl-L-Arginine 4-Methylcoumaryl-7-Amide
CAS No.	155575-02-3
Molecular Formula	C₄₀H₆₈N₁₆O₁₀
Molecular Weight	933.09 g/mol
Synonyms	pGlu-Arg-Thr-Lys-Arg-AMC; Pyroglutamyl-Arginyl-Threonyl-Lysyl-Arginine-7-amido-4-methylcoumarin; Fluorogenic Substrate for Trypsin-like Proteases; Boc-PyroGlu-Arg-Thr-Lys-Arg-AMC; AMC-labeled Peptide Substrate; Protease Substrate 155575-02-3; 4-Methylcoumaryl-7-Amide (AMC) Peptide Derivative
EINECS	Contact for details

Quality Control

Our L-Pyroglutamyl-L-Arginyl-L-Threonyl-L-Lysyl-L-Arginine 4-Methylcoumaryl-7-Amide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity verification, to meet the stringent requirements of research and diagnostic applications. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Fluorogenic Activity	Functional activity confirmed by enzymatic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.