Description

Calreticulin is a multifunctional, calcium-binding chaperone protein primarily located in the endoplasmic reticulum. Its role in cellular processes such as calcium homeostasis, protein folding, and immune response modulation makes it a critical reagent for advanced life science research. This high-purity protein is essential for researchers and developers in the fields of immunology, cell biology, and molecular diagnostics. The product is supplied as a lyophilized powder, ensuring stability and ease of reconstitution for experimental use.

Application

• Biomedical Research: Used as a key antigen and reagent in studies of autoimmune diseases, cancer immunology, and apoptosis.
• Diagnostic Development: Serves as a critical component in the development of immunoassays and diagnostic kits for detecting anti-calreticulin antibodies.
• Cell Signaling Studies: Employed to investigate endoplasmic reticulum stress pathways, calcium signaling, and phagocytosis.
• Protein Interaction Analysis: Utilized in pull-down assays, co-immunoprecipitation, and other techniques to study protein-protein interactions within the ER.
• Vaccine Research: Investigated for its role as an immunogenic "eat-me" signal in cancer immunotherapy and vaccine adjuvants.
• Quality Control for Biologics: Acts as a standard or control in the quality assessment of therapeutic proteins produced in mammalian cell systems.

Basic Information

Product Name	Calreticulin
CAS No.	154837-41-9
Molecular Formula	Contact for details
Molecular Weight	~46 kDa (approx., varies by species and post-translational modifications)
Synonyms	CRT; Calregulin; Endoplasmic Reticulum Resident Protein 60; ERp60; HACBP; cC1qR; Ro/SS-A antigen; Autoantigen Ro; Sicca syndrome antigen A; CRP55; CaBP3; Calreticulin Precursor
EINECS	Contact for details

Quality Control

Our Calreticulin is produced and tested under stringent conditions to ensure the highest standards of purity and biological activity for research applications. Each batch is characterized using advanced analytical techniques, including SDS-PAGE, Western Blot, and functional activity assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, endotoxin levels, and biological activity is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep the lyophilized powder at -20°C or below. Upon reconstitution, aliquot the solution to avoid repeated freeze-thaw cycles and store at -80°C. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Identification (Western Blot)	Positive
Protein Content (BCA)	≥ 90% of stated weight
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Confirmed by calcium-binding or cell-based assay
Solubility	Soluble in PBS or Tris buffer, pH 7.2-7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.