Description

Rab7 Protein is a purified recombinant protein belonging to the Ras superfamily of small GTPases, specifically identified by CAS NO 152989-05-4. This protein plays a critical role in regulating endocytic trafficking, lysosomal biogenesis, and autophagy, making it an essential reagent for cellular and molecular biology research. It is primarily utilized by researchers and scientists in the fields of cell biology, neuroscience, immunology, and drug discovery for in vitro studies, assay development, and target validation.

Application

• Biochemical Research: Used as a standard or substrate in enzymatic activity assays (GTPase activity) and protein-protein interaction studies.
• Drug Discovery & Screening: Serves as a target protein in high-throughput screening (HTS) campaigns to identify novel modulators of Rab7 function for therapeutic applications.
• Antibody Production & Validation: Acts as a high-purity antigen for the generation and specificity testing of monoclonal and polyclonal antibodies.
• Cell Signaling Studies: Employed in pull-down assays and western blotting to investigate its role in vesicular transport, autophagy, and neurodegenerative disease pathways.
• Diagnostic Development: Used as a reference material in the development of diagnostic kits targeting autophagy-related disorders.
• Academic & Institutional Research: Fundamental tool for studying lysosome function, pathogen infection mechanisms, and aging at leading research institutions.

Basic Information

Product Name	Rab7 Protein
CAS No.	152989-05-4
Molecular Formula	Contact for details
Molecular Weight	~23-25 kDa (approx., dependent on construct and tags)
Synonyms	Ras-related protein Rab-7a
RAB7A
RAB7
Ras-associated protein RAB7
Recombinant Human RAB7A Protein
GTP-binding protein RAB7
Recombinant Rab7 GTPase
EINECS	Contact for details

Quality Control

Our Rab7 Protein is produced under stringent conditions to ensure high purity, activity, and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including SDS-PAGE, Western Blot, and functional activity assays. A detailed Certificate of Analysis (COA) is provided, confirming identity, purity (>95% by SDS-PAGE), concentration, endotoxin level, and biological activity. Our quality system is designed to support research reproducibility and compliance with relevant guidelines.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive). Thaw on ice and keep on ice when in use. The buffer composition should be confirmed from the specific COA.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	> 95%
Concentration	Provided on COA (typically 0.1-1.0 mg/mL)
Formulation Buffer	Tris, NaCl, Glycerol, DTT (or similar stabilizing buffer)
Identification (Western Blot)	Positive for anti-Rab7 antibody
Biological Activity	GTPγS binding assay or effector pull-down (Data on COA)
Endotoxin Level	< 1.0 EU/μg (LAL assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.