Description

4-Nitro-Z-Gly-Cys(7-Nitro-Benzo[2,1,3]Oxadiazol-4-Yl)-Gly-Oh is a specialized, high-purity peptide derivative featuring a nitrobenzoxadiazole (NBD) fluorophore, making it a critical tool for advanced biochemical research and diagnostics. This compound matters because it serves as a highly sensitive fluorescent probe and building block for studying enzyme activity, protein interactions, and cellular processes. It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions focused on proteomics, fluorescence labeling, and assay development.

Application

• Fluorescent Labeling Reagent: Used for the covalent labeling of peptides, proteins, and other biomolecules to enable detection and imaging.
• Protease & Enzyme Substrate: Acts as a sensitive fluorogenic substrate for studying the kinetics and inhibition of cysteine proteases and other hydrolytic enzymes.
• Bioconjugation & Probe Synthesis: Serves as a key intermediate in the synthesis of more complex fluorescent probes and diagnostic conjugates.
• Cell Biology & Imaging: Employed in fluorescence microscopy and flow cytometry to track cellular uptake, localization, and metabolic pathways.
• Diagnostic Assay Development: Integrated into the development of high-sensitivity ELISA, FRET, and other bioassay platforms.
• Pharmaceutical Research: Used in drug discovery for target validation, high-throughput screening (HTS), and mechanistic studies of therapeutic candidates.

Basic Information

Item	Details
Product Name	4-Nitro-Z-Gly-Cys(7-Nitro-Benzo[2,1,3]Oxadiazol-4-Yl)-Gly-Oh
CAS No.	152390-46-0
Molecular Formula	C22H20N6O10S
Molecular Weight	560.49 g/mol
Synonyms	N-[(Benzyloxy)carbonyl]glycyl-N-[2-[[(4-nitro-2,1,3-benzoxadiazol-7-yl)thio]methyl]-2-(glycylamino)ethyl]-L-cysteinamide; Z-Gly-Cys(NBD)-Gly-OH; NBD-Cysteine Peptide Derivative; 4-Nitro-Z-Gly-Cys(NBD)-Gly-OH; Fluorescent Cysteine Peptide; NBD-labeled Peptide; S-(7-Nitro-2,1,3-benzoxadiazol-4-yl)cysteine derivative; N-Cbz-Gly-Cys(NBD)-Gly-OH
EINECS	Contact for details

Quality Control

Every batch of 4-Nitro-Z-Gly-Cys(7-Nitro-Benzo[2,1,3]Oxadiazol-4-Yl)-Gly-Oh is manufactured under strict quality management systems. We provide comprehensive analytical documentation, including HPLC purity profiles, mass spectrometry (MS) confirmation, and NMR data to ensure identity and consistency. Certificates of Analysis (COA) are available for each lot, detailing specifications for purity, related substances, and residual solvents, supporting compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, light, or moisture will lead to degradation. For long-term stability, store in aliquots under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%; Total impurities ≤ 5.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.