Description

Sulfosuccinimidyl 7-Amino-4-Methylcoumarin-3-Acetate is a highly reactive, amine-reactive fluorescent labeling reagent. This compound is critical for researchers and manufacturers who require the covalent attachment of a bright, blue-fluorescent coumarin tag to proteins, peptides, and other amine-containing biomolecules. It is primarily used in the life sciences and diagnostics industries for applications such as fluorescence microscopy, flow cytometry, and assay development, enabling the tracking and quantification of biological processes.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amines (lysine residues) for creating fluorescent probes used in immunofluorescence, Western blotting, and cellular imaging.
• Peptide Tagging for Proteomics Research: Modification of synthetic peptides to study protein-protein interactions, localization, and turnover rates.
• Development of Fluorescence-Based Assays (FLISA): Creating sensitive detection reagents for enzymatic activity assays, receptor binding studies, and high-throughput screening (HTS).
• Preparation of Molecular Probes for Flow Cytometry: Labeling cell surface proteins or intracellular targets for analysis and sorting of cell populations.
• Bioconjugation for Diagnostic Kits: Manufacturing key components for in vitro diagnostic (IVD) devices that rely on fluorescent detection.
• Material Science & Polymer Chemistry: Functionalizing amine-modified surfaces, nanoparticles, or polymers with a fluorescent marker for tracking and characterization.

Basic Information

Product Name	Sulfosuccinimidyl 7-Amino-4-Methylcoumarin-3-Acetate
CAS No.	152305-86-7
Molecular Formula	C20H17N2O9S
Molecular Weight	461.42 g/mol
Synonyms	AMCA-X, SE; 7-Amino-4-methylcoumarin-3-acetic acid, succinimidyl ester; AMCA succinimidyl ester; AMCA-OSu; 3-Acetyl-7-amino-4-methylcoumarin N-succinimidyl ester; Sulfo-NHS-AMCA; Fluorescent amine-reactive probe; N-Succinimidyl 7-Amino-4-methylcoumarin-3-acetate sulfonate
EINECS	Contact for details

Quality Control

Our Sulfosuccinimidyl 7-Amino-4-Methylcoumarin-3-Acetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) detailing purity, fluorescence properties, and performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~19,000 cm⁻¹M⁻¹ (at 345 nm)
Fluorescence Emission Max	~445 nm (Blue)
Solubility	Soluble in DMF, DMSO; sparingly soluble in water
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.