Description

Orf138 Protein is a specific mitochondrial protein encoded by the *orf138* gene, widely studied for its role in cytoplasmic male sterility (CMS) in plants, particularly in *Brassica* species. This protein is a critical research tool for plant biologists and geneticists investigating fertility restoration mechanisms and hybrid seed production. It is essential for applications in agricultural biotechnology, plant breeding programs, and advanced molecular biology research focused on mitochondrial-nuclear interactions.

Application

• Plant Cytoplasmic Male Sterility (CMS) Research: Fundamental studies on the molecular mechanisms of pollen abortion and sterility in crops like radish and rapeseed.
• Hybrid Seed Production Technology: Enabling the development and optimization of efficient breeding systems for high-yield hybrid varieties.
• Mitochondrial Gene Expression Analysis: Used as a model protein to study mitochondrial genome function, protein import, and organelle-nucleus communication.
• Fertility Restorer Gene Studies: Investigating the interaction between the *Rfo* restorer gene product and the Orf138 protein to understand fertility restoration pathways.
• Agricultural Biotechnology Development: Supporting R&D for creating novel male-sterile lines to improve crop breeding efficiency and genetic diversity.
• Protein-Protein Interaction Assays: Serving as a target in studies to identify interacting partners within the mitochondrial membrane.

Basic Information

Product Name	Orf138 Protein
CAS No.	152206-61-6
Molecular Formula	Contact for details
Molecular Weight	Approx. 15.8 kDa (Theoretical)
Synonyms	ORF138; Mitochondrial ORF138 protein; CMS-associated protein; *orf138* gene product; Brassica CMS protein; Radish mitochondrial sterility protein; pPolCMS; Male sterility-associated protein; Fertility restorer target protein
EINECS	Contact for details

Quality Control

Our Orf138 Protein is produced and handled under stringent conditions to ensure batch-to-batch consistency and biological activity for research applications. Quality is verified through a combination of analytical techniques including SDS-PAGE, Western Blot, and Mass Spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, and buffer composition is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted protein aliquots should be stored at -80°C to prevent degradation. This product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Western Blot)	Consistent with Orf138 protein
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	As per COA (e.g., 0.5-1.0 mg/mL after reconstitution)
Biological Activity	Confirmed by in vitro binding assay (Contact for details)
Endotoxin Level	< 1.0 EU/µg (where applicable)
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.