Description

Phosphorylase Kinase β-Subunit Fragment (420-436) is a synthetic peptide fragment corresponding to a specific region of the regulatory β-subunit of phosphorylase kinase. This compound is a critical research tool for studying enzyme regulation, signal transduction pathways, and the molecular mechanisms of glycogen metabolism. It is primarily utilized by researchers and pharmaceutical developers in biochemical research, proteomics, and drug discovery programs targeting metabolic disorders.

Application

• Biochemical Research: Used as a substrate, inhibitor, or antigenic peptide in studies of phosphorylase kinase activity and regulation.
• Signal Transduction Studies: Essential for investigating calcium/calmodulin-dependent protein kinase pathways and glycogenolytic cascades.
• Antibody Production: Serves as a hapten for generating sequence-specific polyclonal or monoclonal antibodies.
• Drug Discovery & Screening: Employed in high-throughput assays to identify and characterize novel modulators of kinase function.
• Proteomics & Protein Interaction Mapping: Used in pull-down assays and mass spectrometry to map binding partners and protein complexes.
• Enzyme Kinetics & Mechanism: Facilitates detailed kinetic analysis to understand the role of the β-subunit in holoenzyme assembly and activation.

Basic Information

Item	Detail
Product Name	Phosphorylase Kinase β-Subunit Fragment (420-436)
CAS No.	150829-21-3
Molecular Formula	C84H138N24O23S
Molecular Weight	1884.2 g/mol
Synonyms	Phosphorylase Kinase Beta Subunit (420-436); PhK β-Subunit Fragment (420-436); Glycogen Phosphorylase Kinase Beta Chain Peptide (420-436); PHKB (420-436); Synthetic Phosphorylase Kinase Peptide; Calmodulin-Binding Domain Peptide (PhK Beta); Research Peptide 150829-21-3
EINECS	Contact for details

Quality Control

Our peptide products are manufactured under strict quality control protocols to ensure batch-to-batch consistency and high biological activity. Each lot is characterized by multiple analytical techniques, and a comprehensive Certificate of Analysis (COA) is provided, detailing purity, sequence verification, and mass confirmation. We adhere to standards suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (HPLC)	≥ 95%
Identification (Mass Spec)	Consistent with theoretical molecular weight
Peptide Content	≥ 70%
Amino Acid Sequence	H-Lys-Arg-Gln-Gln-Ile-Ser-Val-Arg-Phe-Leu-Gly-Lys-Lys-Lys-Asn-Ala-OH
Single-Letter Sequence	KRQQISVRFLGKKKNA
Solubility	Soluble in water or aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.