Description

5(6)-Carboxytetramethylrhodamine Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in bioanalytical chemistry. Its core value lies in its ability to form stable amide bonds with primary amines, enabling the sensitive detection and quantification of biomolecules. This compound is essential for researchers and manufacturers in the fields of bioconjugation, flow cytometry, immunoassay development, and fluorescence microscopy. The product is supplied under strict quality control to ensure batch-to-batch consistency for critical life science applications.

Application

• Antibody and Protein Labeling: Conjugation to primary amines (e.g., lysine residues) for creating fluorescent probes used in immunoassays (ELISA, Western Blot) and immunohistochemistry.
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides and nucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cell and Particle Tracking: Fluorescent labeling of microspheres, nanoparticles, and cell surface proteins for flow cytometry and cellular imaging studies.
• Bioconjugation for Diagnostics: Key reagent in the development of fluorescent diagnostic kits and biosensors for clinical and research use.
• Pharmacokinetic Studies: Used to label peptides, proteins, and other therapeutic agents to track their distribution and metabolism in biological systems.
• Material Science Functionalization: Introducing fluorescent tags onto polymers, surfaces, or nanomaterials for analytical and sensing purposes.

Basic Information

Item	Detail
Product Name	5(6)-Carboxytetramethylrhodamine Succinimidyl Ester
CAS No.	150408-83-6
Molecular Formula	C32H31N3O7
Molecular Weight	569.61 g/mol
Synonyms	5(6)-TAMRA, SE; 5(6)-Carboxy-tetramethylrhodamine N-succinimidyl ester; 6-TAMRA, SE; TAMRA NHS ester; 5(6)-Carboxy-tetramethylrhodamine succinimidyl ester; N-Succinimidyl ester of 5(6)-carboxytetramethylrhodamine; Mixed isomers of 5- and 6-carboxytetramethylrhodamine succinimidyl ester; X-Rhodamine, SE (mixed isomers)
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxytetramethylrhodamine Succinimidyl Ester is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) detailing purity, absorbance/fluorescence properties, and isomer ratio are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation and hydrolysis. For long-term stability, keep the material under inert atmosphere (argon or nitrogen) after opening.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0% (sum of 5- and 6- isomers)
Absorption Maximum (λmax)	540 - 555 nm
Emission Maximum	565 - 580 nm
Extinction Coefficient (ε)	≥ 90,000 cm⁻¹M⁻¹
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.