Description

5(6)-(n-Succinimidyloxycarbonyl)-3',6',O,O'-Diacetylfluorescein is a highly reactive, amine-reactive derivative of fluorescein, designed for the efficient labeling of proteins, peptides, and other biomolecules. This compound is critical for researchers and manufacturers requiring stable, fluorescent conjugates for sensitive detection and analysis. It is primarily used in the biotechnology, pharmaceutical, and diagnostic industries for applications such as immunoassay development, fluorescence microscopy, and flow cytometry.

Application

• Fluorescent Labeling of Biomolecules: Primary application for covalently tagging primary amines on proteins, antibodies, and peptides to create stable fluorescent probes.
• Immunoassay Development: Used to prepare fluorescently-labeled antibodies and antigens for ELISA, Western blotting, and other immunoassay techniques.
• Cellular Imaging & Microscopy: Enables the tracking and visualization of cellular components and processes in fluorescence microscopy and live-cell imaging.
• Flow Cytometry: Essential for creating fluorescent markers to detect and sort specific cell populations based on surface or intracellular antigens.
• Diagnostic Kit Manufacturing: A key reagent in the production of fluorescent-based diagnostic tests and kits for clinical and research use.
• Bioconjugation & Probe Synthesis: Serves as a fundamental building block for synthesizing custom fluorescent probes and molecular beacons.
• Protein Interaction Studies: Facilitates fluorescence resonance energy transfer (FRET) and other proximity-based assays to study protein-protein interactions.

Basic Information

Product Name	5(6)-(n-Succinimidyloxycarbonyl)-3',6',O,O'-Diacetylfluorescein
CAS No.	150347-59-4
Molecular Formula	C33H23NO12
Molecular Weight	625.54 g/mol
Synonyms	5(6)-FAM, SE; 5(6)-Carboxyfluorescein N-succinimidyl ester, Diacetate; Fluorescein-5(6)-isothiocyanate analogue, NHS ester; 3',6'-Diacetylfluorescein-5(6)-carboxamidocaproic acid N-succinimidyl ester; 5(6)-FAMSE; 6-FAM, SE Diacetate; Fluorescein Diacetate NHS Ester; 5-(and-6)-Carboxyfluorescein N-succinimidyl ester, 3',6'-Diacetate
EINECS	Contact for details

Quality Control

Our 5(6)-(n-Succinimidyloxycarbonyl)-3',6',O,O'-Diacetylfluorescein is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive life science applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing specific results for purity, absorbance, and fluorescence properties is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Absorbance (λmax)	~495 nm (in suitable buffer)
Emission (λmax)	~520 nm (in suitable buffer)
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.