Description

5(6)-Carboxynaphthofluorescein n-Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent derived from the naphthofluorescein dye scaffold. This compound matters for its superior photostability and long-wavelength fluorescence emission, which is critical for reducing background interference in complex biological matrices. It is primarily needed by researchers and manufacturers in the life sciences sector for developing sensitive diagnostic assays, advanced cell imaging probes, and high-throughput screening applications.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to primary amines on antibodies, proteins, peptides, and oligonucleotides for detection and quantification.
• Diagnostic Assay Development: Key reagent in the synthesis of fluorescent tracers and probes for ELISA, lateral flow assays, and other immunoassay formats.
• Cell and Tissue Imaging: Preparation of fluorescent conjugates for confocal microscopy, flow cytometry, and in vivo imaging studies.
• Bioconjugation & Chemical Biology: Tool for studying protein-protein interactions, receptor labeling, and intracellular tracking due to its bright, stable signal.
• High-Throughput Screening (HTS): Used to label targets in drug discovery platforms for fluorescence-based detection.
• Material Science: Functionalization of nanoparticles and polymeric materials with fluorescent tags for tracking and analysis.

Basic Information

Product Name	5(6)-Carboxynaphthofluorescein n-Succinimidyl Ester
CAS No.	150347-58-3
Molecular Formula	C34H19NO9
Molecular Weight	585.52 g/mol
Synonyms	5(6)-Carboxy-2',7'-dichloro-4',5'-dichlorofluorescein N-succinimidyl ester; 5(6)-Carboxy-2',7'-dichloro-4',5'-dichlorofluorescein, SE; Naphthofluorescein isothiocyanate analogue, NHS ester; 5(6)-Naphthofluorescein carboxylic acid succinimidyl ester; 6-Carboxynaphthofluorescein N-succinimidyl ester; 5-Carboxynaphthofluorescein N-succinimidyl ester; Mixed 5- and 6-isomers, NHS ester; Naphthofluorescein-NHS
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxynaphthofluorescein n-Succinimidyl Ester is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and isomer ratio, to ensure consistency and performance for critical research and diagnostic applications. Certificates of Analysis (COA) detailing identity, purity, and other relevant specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Isomer Ratio (5-/6-)	Approx. 1:1 mixture
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.