Description

Flight Muscle Protein, Act88F is a specialized recombinant protein derived from the flight muscle of Drosophila melanogaster, specifically the actin isoform Act88F. This high-purity protein is a critical research tool for studying muscle development, function, and related genetic disorders. It is essential for biotech and pharmaceutical R&D teams focused on molecular biology, structural biology, and proteomics. The product is supplied as a lyophilized powder to ensure stability and ease of reconstitution for experimental use.

Application

• Biomedical Research: A key reagent for in vitro studies of actin filament assembly, dynamics, and protein-protein interactions within the sarcomere.
• Drug Discovery: Used in high-throughput screening assays to identify novel compounds that modulate actin function for potential therapeutic applications in muscular diseases.
• Structural Biology: Serves as a substrate for X-ray crystallography, cryo-electron microscopy, and NMR studies to elucidate the three-dimensional structure of flight muscle actin.
• Proteomics & Biomarker Development: Employed as a standard or antigen in mass spectrometry and immunoassay development for muscle-specific protein analysis.
• Genetic Disorder Modeling: Facilitates functional studies to understand mutations in actin genes and their role in congenital myopathies and nemaline myopathy.
• Educational & Diagnostic Kit Manufacturing: Used as a purified component in advanced life science educational kits and in the development of diagnostic reagents.

Basic Information

Product Name	Flight Muscle Protein, Act88F
CAS No.	149954-56-3
Molecular Formula	Contact for details
Molecular Weight	~42 kDa (Approximate)
Synonyms	Act88F Actin; Drosophila Actin 88F; Flight Muscle Actin; Actin, muscle-specific isoform 88F; Recombinant Act88F Protein; IFM Actin; Insect Flight Muscle Actin; Actin 88F (Drosophila melanogaster)
EINECS	Contact for details

Quality Control

Our Flight Muscle Protein, Act88F is produced under stringent conditions to ensure the highest standards of purity and biological activity. Each batch undergoes rigorous quality testing, including SDS-PAGE, Western Blot, and functional activity assays, to ensure compliance with research-grade specifications. Certificates of Analysis (COA) detailing purity, concentration, endotoxin levels, and biological activity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store the lyophilized powder at -20°C or below for long-term stability. Upon reconstitution, aliquot and store at -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~42 kDa
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90%
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Confirmed by in vitro polymerization assay
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.