Description

Oct3A Protein CAS NO 149086-18-0 is a highly purified recombinant protein, specifically the POU domain, class 5, transcription factor 1 (POU5F1), also known as Octamer-binding transcription factor 3/4 (Oct-3/4). This protein is a critical research-grade reagent for studying the molecular mechanisms of pluripotency, stem cell biology, and cellular reprogramming. It is essential for scientists and R&D teams in the fields of regenerative medicine, developmental biology, and oncology research, where precise control and understanding of gene expression networks are paramount.

Application

• Stem Cell Research: Used in studies of pluripotency maintenance, self-renewal, and differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs).
• Cellular Reprogramming: Acts as a key transcription factor in the Yamanaka factors cocktail for generating iPSCs from somatic cells.
• Gene Regulation Studies: Employed in Electrophoretic Mobility Shift Assays (EMSAs), Chromatin Immunoprecipitation (ChIP), and reporter gene assays to investigate DNA-binding and transcriptional activation.
• Protein-Protein Interaction Analysis: Serves as a bait or prey protein in pull-down assays, co-immunoprecipitation (Co-IP), and yeast two-hybrid screens to identify binding partners.
• Antibody Validation & Specificity Testing: Used as a positive control antigen to validate the specificity of anti-Oct3/4 antibodies in Western blotting and immunohistochemistry.
• Drug Discovery & Toxicology: Applied in high-throughput screening assays to identify compounds that modulate Oct3/4 activity for potential cancer therapeutics or regenerative medicine applications.
• Biomarker Research: Investigated as a potential biomarker for certain germ cell tumors and cancers with stem-like properties.

Basic Information

Product Name	Oct3A Protein
CAS No.	149086-18-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Oct-3/4 Protein; POU5F1 Protein; POU Domain, Class 5, Transcription Factor 1; Octamer-Binding Transcription Factor 3; Octamer-Binding Transcription Factor 4; OTF-3; OTF-4; OCT3; OCT4
EINECS	Contact for details

Quality Control

Our Oct3A Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, confirmation of identity by mass spectrometry, and functional validation in DNA-binding assays. Certificates of Analysis (COA) detailing purity, concentration, buffer composition, and specific activity are available upon request to support your research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed properly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear, colorless to slightly opalescent solution
Purity (SDS-PAGE)	≥ 95%
Identification (Mass Spec)	Conforms
Protein Concentration	≥ 0.5 mg/mL
Endotoxin Level	< 1.0 EU/µg
Biological Activity (EMSA)	Active
Buffer	Specified phosphate or Tris-based buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.