Description

Nup116 Protein, S Cerevisiae CAS NO 148997-72-2 is a highly purified nucleoporin protein derived from *Saccharomyces cerevisiae* (Baker's yeast), a key component of the nuclear pore complex (NPC). This protein is essential for research into nucleocytoplasmic transport, nuclear envelope structure, and the molecular mechanisms of cellular transport. It is a critical reagent for molecular biologists, biochemists, and pharmaceutical researchers focused on gene expression regulation, virology, and the development of targeted therapies. The product is supplied with guaranteed purity and biological activity for reproducible experimental results.

Application

• Biochemical Research: Used as a standard or substrate in studies of nuclear import and export mechanisms.
• Structural Biology: Essential for in vitro reconstitution assays to study the architecture and assembly of the nuclear pore complex.
• Drug Discovery: Employed in high-throughput screening (HTS) platforms to identify compounds that modulate nucleocytoplasmic transport, relevant for cancer and antiviral research.
• Protein-Protein Interaction Studies: Serves as bait or prey in pull-down, co-immunoprecipitation (Co-IP), or yeast two-hybrid assays to map interaction networks within the NPC.
• Antibody Production & Validation: Acts as a high-purity antigen for generating and characterizing specific antibodies against nucleoporins.
• Educational & Diagnostic Development: Used in advanced life science training and as a component in developing diagnostic tools for diseases linked to NPC dysfunction.

Basic Information

Product Name	Nup116 Protein, S Cerevisiae
CAS No.	148997-72-2
Molecular Formula	Contact for details
Molecular Weight	~116 kDa (Theoretical)
Synonyms	Nucleoporin NUP116; NUP116; Nuclear pore protein NUP116; NUP116 Yeast; S. cerevisiae Nucleoporin 116; Nuclear pore complex protein Nup116p; FG-nucleoporin Nup116; Yeast Nup116 Protein; Recombinant S. cerevisiae Nup116
EINECS	Contact for details

Quality Control

Our Nup116 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and size-exclusion chromatography, functional activity assays, and endotoxin level checks. Certificates of Analysis (COA) detailing purity, concentration, buffer composition, and specific activity are available upon request, supporting compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Source	Recombinant S. cerevisiae
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Concentration	≥ 0.5 mg/mL
Formulation	In specified storage buffer (e.g., Tris, NaCl, Glycerol, DTT)
Endotoxin Level	< 1.0 EU/µg
Biological Activity	Confirmed by functional binding assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.