Description

Nup100 Protein, S Cerevisiae is a highly purified nucleoporin protein derived from the yeast *Saccharomyces cerevisiae*, a key component of the nuclear pore complex (NPC). This protein is essential for fundamental research into nucleocytoplasmic transport, nuclear envelope structure, and cellular compartmentalization. It is a critical reagent for molecular biologists, biochemists, and pharmaceutical researchers focused on gene regulation, virology, and the development of targeted therapies.

Application

• Structural Biology Studies: Used in in vitro reconstitution assays to study the architecture and assembly of the nuclear pore complex.
• Nuclear Transport Research: Essential for investigating the mechanisms of importin/exportin-mediated transport of proteins and RNA across the nuclear envelope.
• Virology & Pathogen Research: Employed to understand how viruses hijack host nuclear transport machinery for replication.
• Drug Discovery Screening: Serves as a target protein in high-throughput screens for compounds that modulate nuclear transport, relevant for cancer and antiviral drug development.
• Biomolecular Interaction Analysis: Used in techniques like Surface Plasmon Resonance (SPR) or pull-down assays to map protein-protein interactions within the NPC.
• Educational & Diagnostic Kit Development: Acts as a standard or component in research kits for teaching or diagnostic applications related to cellular biology.

Basic Information

Product Name	Nup100 Protein, S Cerevisiae
CAS No.	148997-71-1
Molecular Formula	Contact for details
Molecular Weight	~100 kDa (Theoretical)
Synonyms	Nucleoporin Nup100; NUP100; Nuclear pore complex protein Nup100p; NUP100-YEAST; Nuclear pore protein NUP100; Yeast Nup100 protein; Recombinant S. cerevisiae Nup100; NPC component Nup100.
EINECS	Contact for details

Quality Control

Our Nup100 Protein is produced and purified under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous analytical testing, including SDS-PAGE for purity assessment, Western Blot for identity confirmation, and functional activity assays. A comprehensive Certificate of Analysis (COA), detailing purity, concentration, buffer composition, and storage instructions, is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot)	Positive for Nup100
Protein Concentration	≥ 0.5 mg/mL (Lot-specific)
Buffer Composition	Tris, NaCl, Glycerol, DTT (Detailed on COA)
Endotoxin Level	< 1.0 EU/µg (for cell culture grades)
Biological Activity	Functional in binding assays (e.g., with importins)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.