Description

Nup49 Protein, S Cerevisiae CAS NO 148997-70-0 is a highly purified nucleoporin protein derived from the yeast *Saccharomyces cerevisiae*, a key component of the nuclear pore complex (NPC). This protein is essential for fundamental research into nucleocytoplasmic transport, nuclear envelope structure, and the molecular mechanisms of cellular compartmentalization. It is a critical reagent for molecular biologists, biochemists, and pharmaceutical researchers focused on gene regulation, virology, and the development of targeted therapies.

Application

• Structural Biology Studies: Used in biochemical and biophysical assays to study the architecture and assembly of the nuclear pore complex.
• Nuclear Transport Research: Essential for in vitro reconstitution experiments to analyze importin/exportin-mediated cargo transport through the NPC.
• Protein-Protein Interaction Analysis: Serves as a bait or prey protein in pull-down assays, co-immunoprecipitation, and surface plasmon resonance (SPR) to map interaction networks.
• Antibody Production & Validation: Acts as a high-purity antigen for generating and characterizing monoclonal or polyclonal antibodies specific to nucleoporins.
• Drug Discovery Screening: Utilized in target-based screens to identify small molecules or biologics that modulate nuclear transport, relevant for cancer and antiviral research.
• Educational & Diagnostic Kit Development: Provides a standardized component for advanced teaching kits and the development of diagnostic assays for nuclear envelope pathologies.

Basic Information

Product Name	Nup49 Protein, S Cerevisiae
CAS No.	148997-70-0
Molecular Formula	Contact for details
Molecular Weight	~49 kDa (Theoretical)
Synonyms	Nucleoporin Nup49; Nuclear pore protein NUP49; NUP49; YGL100W; Yeast Nucleoporin 49; Nuclear Pore Complex Protein Nup49p; Nup49p; S. cerevisiae Nup49
EINECS	Contact for details

Quality Control

Our Nup49 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and size-exclusion chromatography, functional validation in binding assays, and endotoxin level checks. Certificates of Analysis (COA) detailing purity, concentration, buffer composition, and specific activity are provided with every shipment to support your research compliance.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation. Handle in a controlled environment.

Specification

Item	Specification
Appearance	Clear or slightly opalescent liquid
Purity (SDS-PAGE)	≥ 90%
Concentration	≥ 0.5 mg/mL
Buffer	20 mM Tris-HCl, 150 mM NaCl, pH 7.5
Identification (Mass Spec)	Conforms
Endotoxin Level	< 1.0 EU/µg
Biological Activity (Binding Assay)	Confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.