Description

Cot1 Protein is a highly purified, recombinant protein derived from human DNA, specifically designed for use in molecular biology and cytogenetics. This product is essential for suppressing repetitive DNA sequences during fluorescence in situ hybridization (FISH) experiments, ensuring clear and specific signal detection. It is a critical reagent for cytogeneticists, molecular biologists, and researchers in pharmaceutical and diagnostic laboratories working on genomic analysis, cancer research, and prenatal diagnostics.

Application

• Fluorescence In Situ Hybridization (FISH): Used as a blocking agent to suppress non-specific hybridization of repetitive DNA sequences (e.g., Cot-1 DNA), improving the specificity and signal-to-noise ratio in FISH assays.
• Cancer Cytogenetics: Essential for the analysis of chromosomal abnormalities, gene amplifications, and translocations in solid tumors and hematological malignancies.
• Prenatal and Postnatal Genetic Diagnosis: Employed in clinical FISH tests for the rapid detection of aneuploidies (e.g., trisomy 21) and microdeletion syndromes.
• Genomic Research: Facilitates high-resolution mapping of genes and the study of genomic rearrangements in research settings.
• Microarray-Based CGH (Comparative Genomic Hybridization): Used in sample preparation to block repetitive genomic elements, enhancing the accuracy of copy number variation analysis.
• Pharmaceutical Development: Supports target validation and biomarker discovery by enabling precise visualization of genetic loci in cell-based assays.

Basic Information

Product Name	Cot1 Protein
CAS No.	148294-67-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cot-1 DNA Protein; Human Cot-1 DNA Protein; Cot1 Blocking Reagent; Cot-1 DNA Blocking Agent; Cot1 Human DNA; Repetitive DNA Blocking Protein; FISH Blocking Protein; Chromosome In Situ Suppression Protein
EINECS	Contact for details

Quality Control

Our Cot1 Protein is manufactured under strict quality systems. Each batch is rigorously tested for biological activity in standardized FISH blocking assays, purity via electrophoresis, and the absence of contaminating nucleases. Certificates of Analysis (COA) detailing purity, concentration, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Functional Assay)	Effectively blocks Cot-1 DNA in FISH
Concentration	1 mg/mL (Typical)
Purity (SDS-PAGE)	≥ 95%
Biological Activity	Pass
Endotoxin Level	< 1.0 EU/µg
Nuclease Activity	Not detected
Storage Buffer	Tris-HCl, Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.