Description

Nt-Rab5 Protein CAS NO 147995-31-1 is a recombinant protein corresponding to the N-terminal domain of the Rab5 GTPase, a key regulator of early endosomal trafficking. This high-purity protein is essential for in vitro biochemical and biophysical studies of vesicle transport mechanisms. It is primarily utilized by researchers and developers in the life sciences sector for target validation, assay development, and structural biology applications.

Application

• Biochemical Research: Used as a critical reagent in enzymatic activity assays (GTPase activity) and protein-protein interaction studies to elucidate endocytic pathways.
• Drug Discovery Screening: Serves as a target protein in high-throughput screening (HTS) platforms to identify novel modulators of Rab5 function for therapeutic intervention.
• Structural Analysis: Provides a purified, functional protein domain for X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy studies.
• Diagnostic Development: Employed in the development of immunoassays and biosensors for detecting biomarkers associated with trafficking disorders.
• Cell Signaling Studies: Used in reconstitution experiments to study the role of Rab5 in signal transduction from endosomal membranes.
• Protein Standard: Acts as a quantitative standard in Western blotting, ELISA, and other protein detection methodologies.

Basic Information

Product Name	Nt-Rab5 Protein
CAS No.	147995-31-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Recombinant N-terminal Rab5 Protein; N-terminal RAS-associated protein RAB5; Rab5 N-terminal domain; GTP-binding protein RAB5 Nt-domain; Recombinant Human Rab5 Nt-domain; Rab5(1-184); Early endosome antigen; RAB5A N-terminal fragment
EINECS	Contact for details

Quality Control

Our Nt-Rab5 Protein is manufactured under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to a comprehensive suite of analytical tests including SDS-PAGE, Western Blot, and functional activity assays. A detailed Certificate of Analysis (COA) documenting purity, concentration, endotoxin levels, and specific activity is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Upon reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE & Western Blot)	Single band at expected molecular weight
Purity (SDS-PAGE)	≥ 95%
Protein Content (BCA/A280)	≥ 90% of stated concentration
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity (GTPase Assay)	Contact for details
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.