Description

4-Nitrophenyl o-(2-Acetamido-2-Deoxyglucopyranosyl)-(1-2)-o-(6-o-Methylmannopyranosyl)-(1-6)-Glucopyranoside is a specialized synthetic trisaccharide derivative, primarily utilized as a chromogenic substrate for enzyme activity assays. This compound is critical for researchers and manufacturers requiring precise, high-purity biochemical tools for diagnostic and analytical applications. It is essential for the study and quality control of glycosidase enzymes, particularly in the pharmaceutical, diagnostic, and life science research sectors.

Application

• Enzymatic Substrate for Glycosidases: Acts as a chromogenic substrate for the detection and kinetic study of specific α-glucosidase and α-mannosidase enzymes.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for metabolic disorders, such as lysosomal storage diseases.
• Biochemical Research: A key tool in enzymology for investigating enzyme mechanisms, inhibition, and substrate specificity.
• Pharmaceutical Quality Control: Employed in the assay and potency testing of enzyme-based therapeutics and recombinant proteins.
• High-Throughput Screening (HTS): Suitable for use in automated screening platforms to identify potential enzyme inhibitors or activators for drug discovery.
• Academic Research: Supports fundamental research in glycobiology, carbohydrate chemistry, and molecular biology.

Basic Information

Product Name	4-Nitrophenyl o-(2-Acetamido-2-Deoxyglucopyranosyl)-(1-2)-o-(6-o-Methylmannopyranosyl)-(1-6)-Glucopyranoside
CAS No.	147985-37-3
Molecular Formula	C32H48N2O22
Molecular Weight	812.73 g/mol
Synonyms	4-Nitrophenyl 2-acetamido-2-deoxy-4-O-(6-O-methyl-α-D-mannopyranosyl)-α-D-glucopyranosyl-(1→2)-α-D-glucopyranoside; p-Nitrophenyl-α-D-Glcp-(1→2)-α-D-GlcpNAc-(1→6)-α-D-Manp(6-OMe) Substrate; Chromogenic Trisaccharide Substrate; α-Glucosidase Substrate; Glycosidase Chromogenic Substrate; 4-Nitrophenyl trisaccharide derivative; PNPTrisaccharide; Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl trisaccharide is produced under stringent quality protocols to ensure batch-to-batch consistency and high purity suitable for sensitive biochemical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to ISO 9001 quality management principles, and specifications can be tailored to meet research or cGMP guidelines for diagnostic manufacturing upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term stability, containers should be sealed under inert atmosphere or with desiccant. Allow the product to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Enzymatic Activity	Suitable for use as substrate (performance data on COA)
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.