Description

Zw10 Protein CAS NO 147979-57-5 is a highly purified recombinant protein, specifically the human Zw10 kinetochore protein, which plays a critical role in cell division and mitotic checkpoint signaling. This product is essential for researchers and developers in the life sciences sector requiring high-purity biological reagents for mechanistic studies and assay development. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies focused on cell biology, cancer research, and drug discovery.

Application

• Biochemical Research: Used as a key reagent in studies of the mitotic spindle assembly checkpoint (SAC) and chromosome segregation mechanisms.
• Antigen Production: Serves as a high-purity antigen for the generation of specific antibodies used in immunoblotting (Western blot) and immunofluorescence.
• Protein-Protein Interaction Studies: Employed in pull-down assays, co-immunoprecipitation (Co-IP), and yeast two-hybrid screens to map the interactome of the kinetochore.
• Enzyme Activity Assays: Used in in vitro enzymatic assays to study its role and regulation within the RZZ (Rod-Zw10-Zwilch) complex.
• Diagnostic Development: Acts as a reference standard or control protein in the development of diagnostic kits targeting cell cycle dysregulation.
• Drug Discovery Screening: Incorporated into high-throughput screening (HTS) platforms to identify potential therapeutics that target the mitotic checkpoint for oncology applications.

Basic Information

Product Name	Zw10 Protein
CAS No.	147979-57-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Zw10 Kinetochore Protein; ZW10, Centromere/ Kinetochore Protein, Human; ZW10 Homolog; hZW10; ZW10 Mitotic Checkpoint Protein; DKFZp686O24126; FLJ20315; Zwilch-Kinastrin-ZW10 Complex Subunit ZW10; C20orf172
EINECS	Contact for details

Quality Control

Our Zw10 Protein is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including SDS-PAGE, Western blot analysis, and functional activity assays, to ensure high purity, identity, and biological activity. Certificates of Analysis (COA) detailing purity, concentration, endotoxin levels, and buffer composition are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Clear or slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot)	Positive
Protein Concentration	≥ 0.5 mg/mL
Endotoxin Level	< 1.0 EU/μg
Buffer	Specified phosphate or Tris-based buffer
Biological Activity	Confirmed by functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.