Description

Amphiphysin CAS NO 147954-52-7 is a crucial neuronal protein that plays a fundamental role in clathrin-mediated endocytosis at nerve terminals. Its primary value lies in its function as an adaptor protein, essential for recruiting other components to the synaptic vesicle membrane during the recycling process. This makes it a vital research tool for neuroscientists, cell biologists, and pharmaceutical researchers studying synaptic transmission, neurological disorders, and vesicle trafficking mechanisms.

Application

• Neuroscience Research: Key reagent for investigating the molecular mechanisms of synaptic vesicle endocytosis and recycling.
• Biomarker Studies: Used in research related to paraneoplastic neurological disorders, where anti-amphiphysin antibodies are a significant serological marker.
• Cell Biology Assays: Essential for in vitro studies on clathrin-coated pit formation and membrane dynamics in neuronal and non-neuronal cells.
• Drug Discovery Platforms: Employed in high-throughput screening assays to identify compounds that modulate endocytic pathways, relevant for neurological and oncological drug development.
• Diagnostic Development: Serves as an antigen in immunoassays (ELISA, Western Blot) for the detection of autoantibodies in patient serum.
• Protein-Protein Interaction Studies: Used to study its binding partners like dynamin, synaptojanin, and endophilin to map the endocytic protein network.

Basic Information

Item	Details
Product Name	Amphiphysin
CAS No.	147954-52-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Amphiphysin I; AMPH; Amphiphysin (Human); Neuronal Amphiphysin; Amphiphysin, Human Recombinant; Anti-Amphiphysin Antibody Target Protein; Paraneoplastic Antigen; Amphiphysin, Human; Amphiphysin Protein
EINECS	Contact for details

Quality Control

Our Amphiphysin is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing, including purity assessment via SDS-PAGE and HPLC, identity confirmation, and functional activity checks to ensure consistency and reliability for critical research applications. Certificates of Analysis (COA) detailing purity, concentration, and buffer composition are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE/Western Blot)	Consistent with recombinant Amphiphysin
Purity (SDS-PAGE)	> 90%
Purity (HPLC)	> 95%
Endotoxin Level	< 1.0 EU/µg
Protein Concentration	As per COA (typically 0.1 - 1.0 mg/mL)
Buffer Formulation	PBS or Tris-based buffer, pH 7.0-7.5
Biological Activity	Confirmed by functional binding assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.