Description

Ocrl-1 Protein CAS NO 147883-08-7 is a highly purified, recombinant protein derived from the OCRL1 gene, which encodes an inositol polyphosphate 5-phosphatase enzyme. This protein is a critical research tool for studying Lowe syndrome (oculocerebrorenal syndrome) and related cellular signaling pathways involving phosphoinositide metabolism. It is essential for biochemical research, target validation, and drug discovery programs in academic institutions, pharmaceutical R&D, and biotechnology companies focused on rare genetic disorders and cell biology.

Application

• Enzymatic Activity Studies: Used as a substrate to investigate the 5-phosphatase activity on specific phosphoinositides like PI(4,5)P2 and PI(3,4,5)P3.
• Disease Mechanism Research: A vital reagent for modeling Lowe syndrome and Dent-2 disease to understand the pathophysiology linked to OCRL1 mutations.
• Drug Screening Assays: Employed in high-throughput screening (HTS) platforms to identify potential therapeutic compounds that modulate OCRL-1 enzyme function.
• Protein-Protein Interaction Analysis: Used in pull-down assays, co-immunoprecipitation, and blot overlay experiments to map interactomes involving the OCRL-1 protein.
• Antibody Production & Validation: Serves as a high-purity antigen for generating and characterizing specific monoclonal or polyclonal antibodies.
• Biochemical Reagent: A key component in in vitro reconstitution experiments to study endocytic trafficking, actin cytoskeleton dynamics, and ciliary function.

Basic Information

Product Name	Ocrl-1 Protein
CAS No.	147883-08-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Recombinant OCRL1 Protein; Inositol polyphosphate 5-phosphatase OCRL-1; Lowe oculocerebrorenal syndrome protein; OCRL; OCRL1; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; PI(4,5)P2 5-phosphatase; PIP2 5-phosphatase
EINECS	Contact for details

Quality Control

Our Ocrl-1 Protein is produced under stringent conditions and undergoes rigorous quality testing to ensure high purity, identity, and functional activity. Each batch is analyzed for enzymatic activity, purity via SDS-PAGE and HPLC, and is confirmed to be endotoxin-free. Certificates of Analysis (COA) detailing specific purity, concentration, and activity data are available upon request, supporting compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot)	Positive for OCRL1
Protein Concentration	≥ 0.5 mg/mL
Specific Activity	Contact for details
Endotoxin Level (LAL)	< 1.0 EU/µg
Buffer Composition	Tris, NaCl, Glycerol, DTT, pH 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.