Description

Dbp73D Protein, Drosophila is a highly purified recombinant protein derived from the *Drosophila melanogaster* model organism, with the CAS Registry Number 147757-74-2. This protein is a critical research reagent for studying fundamental biological processes, including chromatin remodeling, gene regulation, and developmental biology. It is essential for molecular biology laboratories, academic research institutions, and biotechnology companies focused on epigenetics and functional genomics.

Application

• Chromatin Biology Research: Used as a substrate or binding partner in assays to study chromatin structure, histone interactions, and nucleosome dynamics.
• Enzymatic Activity Studies: Employed in in vitro biochemical assays to investigate ATPase, helicase, or other enzymatic activities associated with the Dbp73D protein complex.
• Protein-Protein Interaction Mapping: Serves as bait or prey in pull-down assays, co-immunoprecipitation (Co-IP), and yeast two-hybrid screens to map interaction networks.
• Antibody Production & Validation: Acts as a high-purity antigen for the generation and specificity testing of polyclonal or monoclonal antibodies.
• Structural Biology: Provides a pure protein sample for techniques like X-ray crystallography, NMR spectroscopy, or cryo-electron microscopy to determine 3D structure.
• Functional Genomics & Screening: Utilized in high-throughput screening platforms to identify small molecule modulators or genetic interactors.
• Educational & Diagnostic Development: Supports advanced teaching in molecular biology and serves as a component in the development of research-grade diagnostic tools.

Basic Information

Item	Details
Product Name	Dbp73D Protein, Drosophila
CAS No.	147757-74-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DEAD-box protein 73D; Dbp73D; Dmel/CG1474; ATP-dependent RNA helicase Dbp73D; Drosophila DEAD-box helicase 73D; Recombinant Dbp73D; p73D Protein
EINECS	Contact for details

Quality Control

Our Dbp73D Protein, Drosophila is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous analytical testing, including purity assessment by SDS-PAGE and HPLC, confirmation of identity by mass spectrometry, and functional activity assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and storage conditions is provided with every shipment to guarantee it meets the exacting standards required for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed properly after each use. Store according to your institution's guidelines for biological reagents.

Specification

Item	Specification
Appearance	Clear or slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Purity (HPLC)	≥ 95%
Identification (Mass Spec)	Conforms
Protein Concentration	≥ 0.5 mg/mL (Lot-specific)
Endotoxin Level	< 1.0 EU/µg
Biological Activity	Confirmed by functional assay (Lot-specific)
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.