Description

Cdc14 Protein CAS NO 147757-04-8 is a highly purified, recombinant protein belonging to the dual-specificity phosphatase family, crucial for regulating cell cycle progression. This protein is an essential research tool for studying mitotic exit, DNA damage checkpoint recovery, and centrosome duplication in eukaryotic cells. It is primarily utilized by academic, pharmaceutical, and biotechnology research laboratories focused on cell biology, oncology, and drug discovery.

Application

• Biochemical Research: Used as a key enzyme substrate or reagent in kinase-phosphatase interaction studies to elucidate signaling pathways.
• Enzymatic Assay Development: Serves as a critical component in high-throughput screening (HTS) assays for identifying potential therapeutic inhibitors.
• Cell Cycle Studies: Essential for in vitro experiments investigating the mechanisms controlling mitotic exit and cytokinesis.
• Protein-Protein Interaction Analysis: Employed in pull-down, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) studies to map interactomes.
• Antibody Production & Validation: Acts as a pure antigen for generating and testing specific monoclonal and polyclonal antibodies.
• Structural Biology: Provides material for X-ray crystallography and cryo-electron microscopy to determine 3D protein structures.
• Diagnostic Development: Potential use as a reference standard in developing assays for cellular pathway dysregulation.

Basic Information

Product Name	Cdc14 Protein
CAS No.	147757-04-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	CDC14; Cell Division Cycle 14 Protein; Dual Specificity Phosphatase CDC14; Cdc14 Phosphatase; Recombinant Human Cdc14 Protein; Cdc14A/B Isoforms; hCdc14; EC 3.1.3.48
EINECS	Contact for details

Quality Control

Our Cdc14 Protein is produced under stringent conditions to ensure high purity, activity, and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including SDS-PAGE, Western Blot, and functional activity assays. Certificates of Analysis (COA) detailing purity, concentration, specific activity, and endotoxin levels are provided with every shipment to support your research compliance and reproducibility requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is easily oxidized; handle under inert atmosphere when possible and reconstitute only with appropriate, degassed buffers.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 90%
Concentration	≥ 0.5 mg/mL
Specific Activity	Contact for details
Buffer Composition	Tris, NaCl, Glycerol, DTT, pH 8.0
Endotoxin Level	< 1.0 EU/µg
Identification (Western Blot)	Positive

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.