Description

Srb2 Protein CAS NO 147706-77-2 is a high-purity recombinant protein derived from the Saccharomyces cerevisiae Srb2 gene, playing a critical role in transcriptional regulation. This product is essential for researchers and developers in the life sciences sector requiring a reliable and well-characterized protein for mechanistic studies. It is primarily utilized in biochemical research, pharmaceutical development, and as a key reagent in studies of gene expression and mediator complex function.

Application

• Biochemical Research: Used as a fundamental tool for studying the structure and function of the RNA polymerase II mediator complex.
• Transcriptional Regulation Studies: Essential for in vitro assays investigating the mechanisms of eukaryotic gene expression and activation.
• Protein-Protein Interaction Analysis: Serves as a bait or prey protein in pull-down assays, co-immunoprecipitation, and yeast two-hybrid screens.
• Pharmaceutical Target Validation: Employed in high-throughput screening campaigns to identify potential modulators of transcriptional activity for drug discovery.
• Enzyme and Cofactor Studies: Used to investigate the role of mediator subunits in coupling transcription factors with the basal transcriptional machinery.
• Academic and Industrial R&D: A critical component for laboratories focused on molecular biology, genetics, and proteomics research.

Basic Information

Product Name	Srb2 Protein
CAS No.	147706-77-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Srb2; Mediator of RNA polymerase II transcription subunit 2; MED2; SRB2 Yeast Protein; Transcriptional Mediator Subunit Srb2; Recombinant Yeast Srb2 Protein; YGL025C; GAL11-A; Suppressor of RNA Polymerase B 2
EINECS	Contact for details

Quality Control

Our Srb2 Protein is manufactured under stringent conditions to ensure the highest standards of purity and biological activity. Each batch undergoes comprehensive analytical testing, including SDS-PAGE, Western Blot, and functional activity assays. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming identity, purity, and suitability for research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90%
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Confirmed by functional assay
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.