Description

2-Dodecylresorufin is a specialized resorufin derivative, a high-purity chemical compound primarily utilized as a critical reagent and substrate in biochemical research and diagnostic applications. Its value lies in its role as a highly sensitive and specific fluorogenic substrate for measuring the activity of cytochrome P450 enzymes, particularly CYP1A1 and CYP1A2 isoforms. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and contract research organization (CRO) sectors who require reliable tools for drug metabolism studies, toxicology screening, and enzyme inhibition assays.

Application

• Cytochrome P450 Enzyme Activity Assays: Primary fluorogenic substrate for the sensitive detection and quantification of CYP1A1 and CYP1A2 activity in microsomal preparations, recombinant enzymes, and cell-based systems.
• Drug Metabolism and Interaction Studies: Used in high-throughput screening (HTS) platforms to evaluate the potential of new chemical entities to induce or inhibit specific CYP450 enzymes, a critical step in preclinical drug development.
• Toxicological Research: Employed as a biomarker substrate in assays designed to assess the effects of environmental pollutants, pharmaceuticals, and other xenobiotics on hepatic enzyme function.
• Diagnostic Kit Manufacturing: Serves as a key component in the formulation of in vitro diagnostic (IVD) kits for clinical and research laboratories focused on metabolic phenotyping.
• Biochemical Research Reagent: Utilized in fundamental research to study enzyme kinetics, mechanism of action, and the regulation of the aryl hydrocarbon receptor (AhR) pathway.
• Quality Control in Pharmaceutical Development: Applied as a standard or reference compound in analytical methods development and validation for metabolism studies.

Basic Information

Product Name	2-Dodecylresorufin
CAS No.	147662-88-2
Molecular Formula	C24H31NO3
Molecular Weight	381.51 g/mol
Synonyms	Dodecylresorufin; 7-Hydroxy-2-dodecyl-3H-phenoxazin-3-one; Resorufin dodecyl ether; CYP1A1 Substrate; CYP1A2 Substrate; Fluorogenic P450 substrate; 2-n-Dodecylresorufin
EINECS	Contact for details

Quality Control

Our 2-Dodecylresorufin is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, spectral verification (UV-Vis, Fluorescence), and identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are provided to guarantee the material meets the stringent requirements for biochemical and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is light-sensitive (store away from light). For long-term stability, consider storage at -20°C under desiccant.

Specification

Item	Specification
Appearance	Dark red to purple crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (UV-Vis)	Spectrum matches reference
Purity (HPLC)	≥ 98.0%
Fluorescence Properties	Excitation/Emission maxima consistent with standard
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.