Description

Rpob1 Protein is a highly purified recombinant protein derived from the RNA polymerase beta prime subunit, essential for studying bacterial transcription mechanisms. This product is critical for researchers and developers requiring a reliable and consistent reagent for structural biology, enzymology, and drug discovery applications. It serves as a fundamental component for biochemical assays, target validation, and high-throughput screening in the pharmaceutical and biotechnology sectors. Our supply ensures batch-to-batch consistency, meeting the stringent demands of advanced life science research.

Application

• Enzymology Studies: Used as a core substrate or target in assays investigating bacterial RNA polymerase function and inhibition.
• Drug Discovery & Screening: Essential for high-throughput screening (HTS) campaigns to identify novel antibacterial agents targeting transcription.
• Structural Biology: Employed in X-ray crystallography, cryo-electron microscopy (cryo-EM), and NMR studies for protein structure determination.
• Biochemical Assay Development: Serves as a key reagent in developing in vitro transcription assays and studying transcription factors.
• Antibiotic Research: Fundamental for mechanistic studies and resistance profiling of antibiotics that target the bacterial transcription machinery.
• Protein-Protein Interaction Studies: Used to investigate interactions within the RNA polymerase complex and with regulatory factors.

Basic Information

Product Name	Rpob1 Protein
CAS No.	147651-70-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	RNA Polymerase Beta Prime Subunit Protein; Recombinant RpoB1; DNA-Directed RNA Polymerase Subunit Beta'; Transcription Protein RpoB; RNAP Subunit B'; EC 2.7.7.6 (component); Bacterial Transcription Factor Protein
EINECS	Contact for details

Quality Control

Our Rpob1 Protein is produced under stringent quality management systems to ensure the highest standards of purity and biological activity. Each batch is rigorously tested using advanced analytical techniques, including SDS-PAGE, HPLC, and functional activity assays, to guarantee consistency and performance. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, endotoxin levels, and specific activity is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere or desiccated conditions upon opening to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Identification (Mass Spec)	Conforms
Protein Content (BCA)	≥ 90%
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Assay-specific; COA provided
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.