Description

Fk506 Binding Protein-25 CAS NO 147478-69-1 is a high-purity, biologically active protein that specifically binds the immunosuppressant drug FK506 (Tacrolimus). This binding protein is a critical reagent for studying immunosuppressive mechanisms, signal transduction pathways, and drug-protein interactions in molecular biology and immunology research. It is essential for pharmaceutical R&D teams, academic researchers, and diagnostic manufacturers focused on neurobiology, immunology, and the development of novel therapeutic agents.

Application

• Immunosuppressant Research: A key tool for investigating the mechanism of action of FK506 (Tacrolimus) and related calcineurin inhibitors.
• Drug Discovery & Development: Used in high-throughput screening (HTS) assays and binding studies to identify and characterize new immunomodulatory compounds.
• Signal Transduction Studies: Employed to elucidate calcium-dependent signaling pathways in T-cells and neuronal cells.
• Biochemical Assay Standard: Serves as a critical component in ELISA, pull-down assays, and other in vitro diagnostic (IVD) test development.
• Neurobiology Research: Utilized in studies of neuroprotection and neurodegenerative diseases due to its role in neuronal FK506 binding.
• Protein-Protein Interaction Analysis: Acts as a bait or target protein in studies exploring the interactome of immunophilins.

Basic Information

Item	Details
Product Name	Fk506 Binding Protein-25
CAS No.	147478-69-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	FKBP25; FK506-binding protein 25; FKBP-25; FKBP25 immunophilin; Rotamase; PPIase FKBP25; FK506 binding protein 25 kDa; Tacrolimus binding protein 25
EINECS	Contact for details

Quality Control

Our Fk506 Binding Protein-25 is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous purity analysis, including SDS-PAGE and HPLC, and is tested for specific binding activity. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, activity, and endotoxin levels is provided with every shipment to support your research and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted protein aliquots should be stored at -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~25 kDa
Purity (HPLC)	≥ 95%
Specific Binding Activity	Confirmed
Endotoxin Level	< 1.0 EU/μg
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.