Description

Upf1 Protein CAS NO 147477-72-3 is a highly purified, recombinant protein essential for the nonsense-mediated mRNA decay (NMD) pathway, a critical cellular quality control mechanism. This product is vital for researchers and developers in the life sciences sector who require reliable, high-quality reagents for mechanistic studies and assay development. It is specifically designed for use in biochemical research, molecular biology applications, and the development of diagnostic and therapeutic platforms targeting genetic disorders.

Application

• Biochemical Research: Used as a key reagent in in vitro studies to elucidate the molecular mechanisms of the NMD pathway and RNA metabolism.
• Assay Development: Serves as a critical component in the development of high-throughput screening (HTS) assays for drug discovery targeting NMD-related diseases.
• Protein-Protein Interaction Studies: Employed in pull-down assays, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) to map interaction networks within the NMD complex.
• Enzymatic Activity Analysis: Used in functional assays to study the ATPase and helicase activities of the Upf1 protein.
• Antibody Production & Validation: Acts as a pure antigen for the generation and specificity testing of monoclonal and polyclonal antibodies.
• Diagnostic Tool Development: Potential use as a standard or capture protein in diagnostic kits for research into genetic mutations that trigger NMD.

Basic Information

Product Name	Upf1 Protein
CAS No.	147477-72-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Regulator of nonsense transcripts 1; RENT1; UPF1; hUpf1; Nonsense-mediated mRNA decay factor 1; ATP-dependent RNA helicase UPF1; p125
EINECS	Contact for details

Quality Control

Our Upf1 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, functional activity assays, and endotoxin level checks. A comprehensive Certificate of Analysis (COA) detailing identity, purity, concentration, and specific activity is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Concentration	Provided on COA
Biological Activity	Confirmed by functional assay
Buffer Composition	20 mM Tris-HCl, 300 mM NaCl, 10% Glycerol, pH 8.0
Endotoxin Level	< 1.0 EU/µg
Identification (Mass Spec)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.