Description

A-Kinase Anchor Protein 79 (AKAP79) is a crucial scaffolding protein that organizes key signaling molecules, including protein kinase A (PKA), protein kinase C (PKC), and calcineurin, at specific subcellular locations. This precise compartmentalization is essential for orchestrating rapid and localized cellular responses to second messengers like cAMP and calcium. As a vital research tool, it is primarily used by scientists in pharmaceutical R&D, academic biochemistry, and molecular biology laboratories to study intracellular signaling pathways, neuronal function, and cardiac regulation.

Application

• Biochemical Research: Used as a key reagent to study the structure, function, and protein-protein interactions of AKAP signaling complexes.
• Signal Transduction Studies: Essential for investigating cAMP/PKA and calcium/calcineurin signaling pathways in various cell types.
• Neuroscience Research: Applied in studies of synaptic plasticity, neurotransmitter release, and receptor trafficking due to its role in anchoring kinases near postsynaptic densities.
• Cardiovascular Research: Utilized to explore the regulation of ion channels and contractile proteins in cardiac myocytes.
• Drug Discovery Screening: Serves as a target protein in assays designed to identify novel therapeutics that modulate AKAP-mediated signaling for diseases like heart failure and neurological disorders.
• Protein Interaction Mapping: Employed in pull-down assays, co-immunoprecipitation, and yeast two-hybrid screens to discover new binding partners.
• Reconstitution of Signaling Complexes: Used in in vitro systems to rebuild and analyze the activity of multi-enzyme complexes.

Basic Information

Item	Detail
Product Name	A-Kinase Anchor Protein 79
CAS No.	147388-49-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	AKAP79; A Kinase Anchor Protein 79; AKAP 79; Human AKAP79; AKAP 79 kDa; PRKA79; A-kinase anchoring protein 79; cAMP-dependent protein kinase anchoring protein 79
EINECS	Contact for details

Quality Control

Our A-Kinase Anchor Protein 79 is produced and handled under stringent conditions to ensure batch-to-batch consistency and biological activity. Each lot is subjected to rigorous purity analysis and functional characterization. Certificates of Analysis (COA) detailing purity, identity, and activity are available upon request to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~79 kDa
Purity (SDS-PAGE)	≥ 90%
Protein Content	Determined by UV absorbance
Biological Activity	Functional in binding assays (e.g., PKA RII subunit binding)
Endotoxin Level	< 1.0 EU/μg (for cell culture grades)
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.