Description

Calcium-Binding Protein, Entamoeba Histolytica is a purified protein derived from the pathogenic protozoan parasite, crucial for research into its calcium-dependent signaling and virulence mechanisms. This protein matters as a key reagent for developing diagnostic assays and investigating novel therapeutic targets against amoebiasis, a significant global health concern. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in parasitology, infectious disease research, and drug discovery.

Application

• Biochemical Research: Fundamental studies on calcium signaling pathways and protein function in Entamoeba histolytica.
• Diagnostic Development: As a critical antigen or standard in the development and validation of ELISA and other immunoassays for amoebiasis.
• Drug Discovery: Target protein for high-throughput screening (HTS) to identify potential inhibitors that disrupt calcium binding and parasite virulence.
• Vaccine Research: Investigation as a potential vaccine candidate to elicit a protective immune response against infection.
• Antibody Production: Immunogen for generating specific polyclonal or monoclonal antibodies for research and diagnostic use.
• Comparative Proteomics: Analysis of protein structure and function in comparison to calcium-binding proteins in other organisms.

Basic Information

Product Name	Calcium-Binding Protein, Entamoeba Histolytica
CAS No.	147387-39-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	EhCaBP; Entamoeba histolytica Calcium-Binding Protein; Amoebic Calcium-Binding Protein; Calcium Modulator Protein; EhCBP; Amoebiasis Antigen; Parasitic Calcium Sensor Protein
EINECS	Contact for details

Quality Control

Our Calcium-Binding Protein, Entamoeba Histolytica is produced under stringent conditions to ensure high purity and biological activity, suitable for sensitive research applications. Each batch undergoes rigorous quality testing including purity analysis by SDS-PAGE and HPLC, functional activity assays, and confirmation of immunoreactivity. Certificates of Analysis (COA) detailing purity, concentration, and specific test results are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted protein aliquots should be stored at -70°C to -80°C and avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot)	Positive reaction with specific antiserum
Protein Content (BCA/Bradford)	≥ 90% of total weight
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Calcium-binding activity confirmed by fluorescence assay or equivalent
Solubility	Soluble in appropriate aqueous buffers (e.g., PBS, Tris)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.