Description

Nhp1 Protein CAS NO 147386-98-9 is a highly purified recombinant protein of significant interest in molecular biology and biochemical research. This protein plays a crucial role in the study of ribosome biogenesis and nucleolar function, serving as a key reagent for investigating fundamental cellular processes. It is essential for researchers and developers in the fields of academic research, pharmaceutical discovery, and biotechnology who require reliable, high-quality proteins for functional assays, structural studies, and target validation.

Application

• Biochemical Research: Used as a critical reagent in in vitro studies of ribosome assembly and nucleolar dynamics.
• Target Identification & Validation: Employed in screening assays to identify and validate novel therapeutic targets involved in cell growth and proliferation.
• Protein-Protein Interaction Studies: Serves as a bait or prey protein in pull-down assays, co-immunoprecipitation (Co-IP), and yeast two-hybrid screens to map interaction networks.
• Enzyme Activity Assays: Utilized in functional assays to study its role and regulation within the pre-ribosomal complex.
• Antibody Production & Validation: Acts as a high-purity antigen for the generation and specificity testing of polyclonal or monoclonal antibodies.
• Structural Biology: Provides material for X-ray crystallography, NMR spectroscopy, or cryo-electron microscopy to determine 3D structure and mechanism of action.
• Diagnostic Development: Potential use as a component in developing research-use-only (RUO) diagnostic kits for studying related cellular pathways.

Basic Information

Product Name	Nhp1 Protein
CAS No.	147386-98-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NHP1; Nucleolar protein NHP1; Non-histone protein 1; Ribosome biogenesis protein NHP1; Recombinant Nhp1; NHP1 recombinant protein; Yeast Nhp1p homolog; p47 protein
EINECS	Contact for details

Quality Control

Our Nhp1 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis and functional validation, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder or clear solution
Purity (SDS-PAGE)	≥ 95%
Identification (Mass Spec)	Conforms
Endotoxin Level (LAL assay)	< 1.0 EU/µg
Protein Concentration	Provided on COA
Buffer Composition	Provided on COA
Biological Activity	Assay-dependent; provided on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.