Description

Cisplatin-Dna Structure-Specific Recognition Protein CAS NO 147336-28-5 is a high-purity biochemical reagent designed for advanced research into DNA damage and repair mechanisms. This specialized protein is critical for studying the molecular interactions and structural recognition events triggered by cisplatin-DNA adducts, a cornerstone of platinum-based anticancer therapy research. It is an essential tool for pharmaceutical R&D, academic biochemistry, and molecular biology laboratories focused on oncology, pharmacology, and genotoxic stress response pathways.

Application

• Mechanistic Studies in Anticancer Research: Investigating the specific binding and recognition of proteins to cisplatin-modified DNA structures to elucidate drug action and resistance mechanisms.
• DNA Repair Pathway Analysis: Serving as a key reagent in assays designed to identify and characterize proteins involved in nucleotide excision repair (NER) and other DNA damage response pathways.
• Biomolecular Interaction Assays: Utilized in techniques such as Electrophoretic Mobility Shift Assays (EMSA), Surface Plasmon Resonance (SPR), and fluorescence anisotropy to quantify protein-DNA binding affinity and kinetics.
• Structural Biology: Aiding in X-ray crystallography and cryo-electron microscopy studies to determine high-resolution structures of protein-DNA complexes involving platinum lesions.
• Drug Discovery Screening: Used in high-throughput screening platforms to identify novel compounds that modulate the interaction between repair proteins and cisplatin-damaged DNA.
• Diagnostic Development: Potential use in developing diagnostic tools to assess DNA repair capacity in tumor samples, which can predict response to platinum-based chemotherapy.

Basic Information

Product Name	Cisplatin-Dna Structure-Specific Recognition Protein
CAS No.	147336-28-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cisplatin-DNA Adduct Recognition Protein; Platinum-DNA Binding Protein; DNA-Cisplatin Adduct Specific Protein; HMG-Domain Protein (context-specific); Structure-Specific Recognition Protein 1 (SSRP1); FACT Complex Subunit (context-specific); Damage Recognition Protein for Pt-DNA; Cisplatin Lesion-Specific Binding Factor
EINECS	Contact for details

Quality Control

Our Cisplatin-Dna Structure-Specific Recognition Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity assessment via SDS-PAGE and HPLC, functional activity assays, and confirmation of specific binding to cisplatin-modified DNA. Certificates of Analysis (COA) detailing purity, concentration, buffer composition, and functional data are provided with every shipment to support your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single major band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Concentration	As stated on COA (typically 0.1-1.0 mg/mL)
Biological Activity (EMSA/Binding Assay)	Demonstrates specific binding to cisplatin-modified DNA probe
Endotoxin Level	< 1.0 EU/μg (for cell-based applications)
Buffer Composition	Tris-HCl, NaCl, Glycerol, DTT (or similar stabilizing buffer)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.