Description

Green Fluorescent Protein (GFP) is a revolutionary, naturally occurring protein that exhibits bright green fluorescence when exposed to blue to ultraviolet light. This unique property makes it an indispensable molecular biology tool for visualizing and tracking cellular processes in real-time without the need for external dyes or substrates. It is critical for researchers and developers in biotechnology, pharmaceutical discovery, and life sciences, serving as a reporter gene, a fusion tag for protein localization, and a biosensor.

Application

• Reporter Gene Assays: Used to monitor gene expression, promoter activity, and transfection efficiency in live cells.
• Protein Tagging & Localization: Fused to proteins of interest to visualize their subcellular localization, trafficking, and interactions in vivo.
• Biosensor Development: Engineered into fluorescent biosensors to detect ions, metabolites, and signaling events within living cells.
• Cell Biology & Imaging: Enables long-term, non-invasive tracking of cell lineage, migration, and fate in developmental biology and cancer research.
• High-Throughput Screening (HTS): Acts as a fluorescent readout in drug discovery screens for targets involving gene regulation or protein-protein interactions.
• Virology & Microbiology: Used to tag and track viruses and bacteria within host cells and tissues.
• Neuroscience: Employed to label specific neurons and neural circuits for functional imaging studies.

Basic Information

Product Name	Green Fluorescent Protein
CAS No.	147336-22-9
Molecular Formula	C1062H1690N262O337S6
Molecular Weight	~26.9 kDa
Synonyms	GFP; Aequorea victoria Green Fluorescent Protein; GFP (Aequorea victoria); avGFP; Fluorescent Protein, Green; GFP Wild Type; Green Fluorescent Protein (Aequorea victoria); Recombinant Green Fluorescent Protein; rGFP
EINECS	Contact for details

Quality Control

Our Green Fluorescent Protein is produced and tested under stringent conditions to ensure high purity, consistent fluorescence, and biological activity. Each lot is characterized for identity, purity, and functional performance. A comprehensive Certificate of Analysis (COA) detailing specifications such as purity (by SDS-PAGE and HPLC), absorbance/fluorescence spectra, extinction coefficient, quantum yield, and endotoxin levels is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted protein solutions should be aliquoted and stored at -80°C to avoid repeated freeze-thaw cycles. Store under the inert conditions specified on the product label.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (SDS-PAGE)	Single band at ~27 kDa
Purity (HPLC)	≥ 95%
Absorbance Maxima	~395 nm & ~475 nm
Emission Maxima	~509 nm
Extinction Coefficient	Contact for details
Quantum Yield	Contact for details
Fluorescence Intensity	Batch-specific value provided
Endotoxin Level	< 1.0 EU/µg (for cell culture grade)
Biological Activity	Functional in standard reporter assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.