Description

Cd40 Ligand (CAS NO 147205-72-9) is a crucial transmembrane protein that plays a pivotal role in regulating immune cell activation and proliferation. Its primary commercial and research value lies in its function as a key co-stimulatory signal for B-cell and antigen-presenting cell activation, making it an essential reagent in immunology and therapeutic development. This product is primarily needed by biotechnology and pharmaceutical companies engaged in immunotherapy research, vaccine development, and the production of biologics targeting autoimmune diseases and cancers.

Application

• Immunotherapy Research: Used as a critical co-stimulatory molecule in studies of T-cell and B-cell activation pathways for developing novel cancer immunotherapies.
• Vaccine Adjuvant Development: Employed to enhance the immunogenicity of vaccine candidates by providing necessary co-stimulatory signals to dendritic cells and other antigen-presenting cells.
• Autoimmune Disease Studies: Utilized in vitro and in vivo models to investigate the role of the CD40-CD40L pathway in autoimmune disorders like lupus and rheumatoid arthritis.
• Biologics Production: Serves as a key reagent in the development and quality control of monoclonal antibodies and other protein therapeutics that target the CD40 pathway.
• Cell Culture & Expansion: Applied in research-scale T-cell and dendritic cell culture systems to promote activation, survival, and expansion for adoptive cell transfer therapies.
• Diagnostic Assay Development: Used as a standard or coating antigen in the development of ELISA and other immunoassays to detect CD40L or related antibodies.

Basic Information

Product Name	Cd40 Ligand
CAS No.	147205-72-9
Molecular Formula	C254H402N72O78S8
Molecular Weight	Approx. 5830 Da (monomer)
Synonyms	CD154; CD40L; TRAP; gp39; Tumor Necrosis Factor Ligand Superfamily Member 5; TNFSF5; T-cell antigen CD40-L; CD40 Ligand (CD154) Antigen; IMD3; HIGM1
EINECS	Contact for details

Quality Control

Our Cd40 Ligand is produced under stringent conditions to ensure high biological activity and purity, suitable for critical research and development applications. Each batch undergoes comprehensive analytical testing, including SDS-PAGE, HPLC for purity assessment, and functional bioassays to confirm specific activity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, endotoxin levels, sterility (where applicable), and biological potency.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to prevent repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere or desiccant after opening.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (SDS-PAGE)	> 95%
Purity (HPLC)	> 98%
Endotoxin Level	< 1.0 EU/µg
Biological Activity (Bioassay)	Contact for details
Solubility	Soluble in sterile PBS or cell culture medium

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.