Description

Akt1 Protein CAS NO 147205-48-9 is a highly purified, recombinant protein kinase, also known as Protein Kinase B (PKB), which plays a central role in the PI3K/AKT/mTOR signaling pathway. This pathway is critical for regulating essential cellular processes including growth, proliferation, and survival, making Akt1 a key target for biomedical research and therapeutic development. This product is indispensable for researchers and developers in pharmaceutical discovery, cancer biology, metabolic disease research, and high-throughput screening for novel drug candidates.

Application

• Biochemical Research & Assay Development: Used as a key enzyme in kinase activity assays to study signal transduction mechanisms.
• Drug Discovery & Screening: Essential target protein for high-throughput screening (HTS) and validation of potential PI3K/AKT pathway inhibitors for cancer and other diseases.
• Cancer Biology Studies: Employed to investigate the role of Akt1 in tumorigenesis, metastasis, and resistance to chemotherapy.
• Antibody & Diagnostic Kit Production: Serves as a critical antigen for generating specific antibodies and calibrating diagnostic assays.
• Cell Signaling Pathway Analysis: Used in in vitro studies to dissect the components and regulation of the PI3K/AKT/mTOR pathway.
• Enzyme Kinetics & Characterization: Provides a pure, active enzyme source for detailed kinetic studies and inhibitor profiling.

Basic Information

Item	Detail
Product Name	Akt1 Protein
CAS No.	147205-48-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Protein Kinase B (PKB); RAC-PK-alpha; AKT1; AKT1 Kinase; Recombinant Human AKT1 Protein; v-akt Murine Thymoma Viral Oncogene Homolog 1; PKB Alpha; RAC Alpha Serine/Threonine-Protein Kinase
EINECS	Contact for details

Quality Control

Our Akt1 Protein is produced under stringent conditions to ensure high purity, activity, and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including activity assays, purity assessment by SDS-PAGE and HPLC, and identity confirmation. A detailed Certificate of Analysis (COA) documenting purity, specific activity, and storage buffer composition is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle according to standard laboratory safety protocols for biochemical reagents.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	> 90%
Specific Activity	Contact for details
Protein Concentration	Contact for details
Buffer Composition	Specified on COA
Endotoxin Level	< 1.0 EU/µg
Identity (Mass Spec/Western Blot)	Conforms
Biological Activity (Kinase Assay)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.