Description

Splicing Factor Sc35 is a highly purified recombinant protein essential for pre-mRNA splicing research. This product is critical for studying the molecular mechanisms of spliceosome assembly and function, providing researchers with a reliable tool for biochemical and structural analysis. It is primarily utilized by academic, pharmaceutical, and biotechnology laboratories focused on gene expression, RNA processing, and the development of therapeutics targeting splicing-related diseases.

Application

• Biochemical Research: Used as a key component in in vitro splicing assays to reconstitute and analyze spliceosome activity.
• Protein-Protein Interaction Studies: Employed in pull-down, co-immunoprecipitation (Co-IP), and yeast two-hybrid assays to map the spliceosome interactome.
• Structural Biology: Serves as a vital reagent for X-ray crystallography, cryo-electron microscopy (cryo-EM), and NMR studies of splicing complexes.
• Antibody Production & Validation: Acts as a high-purity antigen for generating and characterizing monoclonal or polyclonal antibodies against splicing factors.
• Drug Discovery Screening: Utilized in high-throughput screening (HTS) platforms to identify small molecule modulators of splicing for therapeutic development.
• Diagnostic Development: Applied in the research and development of diagnostic assays for diseases with underlying splicing defects, such as certain cancers and genetic disorders.

Basic Information

Product Name	Splicing Factor Sc35
CAS No.	147153-65-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	SFRS2; Splicing factor, arginine/serine-rich 2; PR264 protein; SC-35 splicing factor; Splicing component 35 kDa protein; SC35; Serine/arginine-rich splicing factor 2; SR splicing factor 2
EINECS	Contact for details

Quality Control

Our Splicing Factor Sc35 is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and functional validation in in vitro splicing assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and activity is provided with every shipment to support your research compliance and documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Protein Concentration	≥ 0.5 mg/mL
Identification (Mass Spec)	Conforms
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Functional in in vitro splicing assays
Buffer	20 mM HEPES, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.