Description

Pumilio Protein, Drosophila is a highly specific RNA-binding protein derived from the model organism *Drosophila melanogaster*. This protein plays a critical role in post-transcriptional gene regulation, particularly in translational repression and mRNA localization during development. It is an essential research reagent for molecular biologists and biochemists studying RNA biology, neurogenesis, and stem cell fate determination. The product is supplied as a purified, research-grade protein suitable for in vitro studies.

Application

• RNA-Protein Interaction Studies: Used in electrophoretic mobility shift assays (EMSAs), RNA pull-down assays, and SELEX to identify and characterize target RNA sequences.
• Translational Control Research: Investigates mechanisms of mRNA translation repression, a key process in cellular differentiation and development.
• Developmental Biology: Serves as a critical tool for studying asymmetric cell division, germline development, and neuronal patterning in model systems.
• Biochemical Assay Development: Acts as a standard or component in high-throughput screening assays for drug discovery targeting RNA-binding proteins.
• Structural Biology: Provides material for X-ray crystallography, NMR spectroscopy, and cryo-EM studies to determine protein-RNA complex structures.
• Proteomics and Interactome Analysis: Used as bait in co-immunoprecipitation (Co-IP) and mass spectrometry to discover novel protein interaction partners.

Basic Information

Product Name	Pumilio Protein, Drosophila
CAS No.	147036-95-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pumilio Protein (Drosophila); Pum Protein; Pumilio homolog; Pum; Pumilio RNA-binding family member; PUF family RNA-binding protein; Pumilio-1; RNA-binding protein pumilio
EINECS	Contact for details

Quality Control

Our Pumilio Protein, Drosophila is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity assessment by SDS-PAGE and functional validation in RNA-binding assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and activity is provided with every shipment to support your research reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed properly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear, colorless to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	≥ 0.5 mg/mL
Identification (Western Blot)	Positive for Pumilio-specific antibody
Biological Activity (EMSA)	Binds specifically to target NRE/ PRE RNA sequence
Buffer	20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH 7.5
Endotoxin Level	< 1.0 EU/µg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.