Description

Cdc27 Protein CAS NO 147015-50-7 is a highly purified recombinant protein, specifically the human cell division cycle 27 homolog, which plays a critical role as a key regulatory subunit of the anaphase-promoting complex/cyclosome (APC/C). This protein is essential for researchers studying the intricate mechanisms of cell cycle regulation, mitosis, and targeted protein degradation. It is primarily utilized in biochemical and pharmaceutical research for applications such as enzyme activity assays, antibody production, and as a critical reagent in drug discovery targeting cell cycle disorders.

Application

• Biochemical Research: Used as a standard or substrate in enzymatic activity assays to study the function and regulation of the APC/C complex.
• Antibody Production & Development: Serves as a high-purity immunogen for generating specific monoclonal or polyclonal antibodies for diagnostic and research tools.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and validate novel small-molecule inhibitors or modulators of the APC/C for cancer therapeutics.
• Protein-Protein Interaction Studies: A vital component in pull-down assays, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) to map interactomes and signaling pathways.
• Cell Cycle & Cancer Research: Critical for in vitro studies aimed at understanding the molecular basis of mitotic exit, genomic stability, and oncogenesis.
• Diagnostic Kit Development: Used as a reference standard in the development of ELISA or other immunoassays for detecting biomarkers related to cell proliferation.

Basic Information

Product Name	Cdc27 Protein
CAS No.	147015-50-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	APC3; Cell Division Cycle Protein 27 Homolog; CDC27Hs; Anaphase-Promoting Complex Subunit 3; hCDC27; p55CDC; CDC27 (Hs); Recombinant Human CDC27 Protein; APC/C Subunit 3
EINECS	Contact for details

Quality Control

Our Cdc27 Protein is produced under stringent conditions to ensure the highest levels of purity and biological activity. Each batch undergoes rigorous quality testing, including analysis for purity, identity, and endotoxin levels, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing specific test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White or off-white lyophilized powder or clear solution
Purity (SDS-PAGE)	≥ 90%
Identification (Mass Spec)	Conforms
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity	Determined by functional assay (e.g., binding assay)
Protein Concentration	As per COA
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.