Description

Ctf4 Transcription Factor CAS NO 146992-06-5 is a high-purity, biologically active protein complex essential for the study of transcription initiation and chromatin remodeling in eukaryotic cells. This product is critical for researchers and developers requiring a reliable and consistent reagent for advanced molecular biology and biochemical assays. It is primarily utilized in academic research, pharmaceutical R&D, and biotechnology applications focused on gene regulation mechanisms.

Application

• Biochemical Research: Used as a key reagent in in vitro transcription assays to study the mechanics of RNA polymerase II initiation and elongation.
• Chromatin Analysis: Essential for investigating the role of the FACT complex in nucleosome reorganization and histone dynamics.
• Drug Discovery Screening: Employed in high-throughput screening platforms to identify potential therapeutics that target transcription factor interactions.
• Protein-Protein Interaction Studies: Serves as a validated bait or prey protein in pull-down, co-immunoprecipitation (Co-IP), and yeast two-hybrid assays.
• Enzyme Activity Assays: Used to measure and characterize the histone chaperone activity associated with the Spt16 subunit.
• Diagnostic Development: Acts as a reference standard in the development of assays for diseases linked to transcription dysregulation.

Basic Information

Product Name	Ctf4 Transcription Factor
CAS No.	146992-06-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	FACT Complex; Facilitates Chromatin Transcription Complex; Spt16-Pob3 Complex (Yeast); SSRP1-SPT16 Heterodimer; Chromatin-specific transcription elongation factor; FACT Heterodimer; hFACT; Ctf4p; Pob3p
EINECS	Contact for details

Quality Control

Our Ctf4 Transcription Factor is produced under stringent conditions to ensure batch-to-batch consistency and biological activity. Each lot undergoes rigorous quality testing, including purity assessment via SDS-PAGE and functional activity validation in standardized transcription assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and specific activity is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	Provided on COA
Buffer Composition	Tris-HCl, Glycerol, NaCl, DTT
Biological Activity	Functional in in vitro transcription assay
Endotoxin Level	< 1.0 EU/µg
Identification (Western Blot)	Positive for Spt16 and Pob3 subunits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.