Description

Ifp53 Protein CAS NO 146888-25-7 is a highly purified recombinant protein derived from the p53 tumor suppressor gene, a critical regulator of cell cycle and apoptosis. This product is essential for advanced life science research, enabling the study of p53-mediated pathways, protein-protein interactions, and cellular response mechanisms. It is a vital reagent for pharmaceutical R&D, academic institutions, and biotechnology companies focused on oncology, molecular biology, and drug discovery.

Application

• Biochemical Research: Used as a standard or substrate in enzymatic assays, binding studies, and functional analysis of the p53 pathway.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify novel compounds that modulate p53 activity for cancer therapeutics.
• Antibody Production & Validation: Serves as a critical antigen for the generation and specificity testing of anti-p53 monoclonal and polyclonal antibodies.
• Diagnostic Development: Utilized in the development and calibration of immunoassays (e.g., ELISA, Western Blot) for p53 detection and quantification.
• Cell Signaling Studies: Applied in vitro to investigate DNA damage response, cell cycle arrest, and apoptotic signaling cascades.
• Protein Interaction Analysis: A key component in pull-down assays, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) to map interactomes.

Basic Information

Product Name	Ifp53 Protein
CAS No.	146888-25-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Recombinant p53 Protein; Tumor Protein p53; TP53; Antigen NY-CO-13; p53 Tumor Suppressor; Phosphoprotein p53; Cellular Tumor Antigen p53; Mutant p53 Protein; FLJ92943
EINECS	Contact for details

Quality Control

Our Ifp53 Protein is manufactured under stringent conditions to ensure high purity, stability, and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including SDS-PAGE, HPLC, and functional activity assays. A detailed Certificate of Analysis (COA) documenting purity, concentration, endotoxin levels, and biological activity is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. Aliquot as recommended upon receipt to maintain optimal activity.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Content	≥ 90% (Bradford/BCA assay)
Endotoxin Level	< 1.0 EU/μg
Biological Activity	Confirmed by functional assay (e.g., DNA binding)
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.