Description

Rap1-Interacting Protein, Yeast is a highly purified recombinant protein derived from *Saccharomyces cerevisiae*, specifically designed for biochemical and cell signaling research. This product is critical for studying the molecular mechanisms of Rap1-mediated pathways, which are fundamental to processes like telomere maintenance, transcription regulation, and cell polarity. It is an essential reagent for molecular biologists, biochemists, and pharmaceutical researchers focused on signal transduction, aging, and oncology research, where precise protein-protein interaction studies are required.

Application

• Biochemical Research: Used as a key reagent in in vitro binding assays to study interactions with Rap1 GTPase and other signaling components.
• Signal Transduction Studies: Essential for reconstituting and analyzing the Rap1 signaling pathway to understand its role in cell growth, differentiation, and oncogenesis.
• Enzyme Kinetics & Characterization: Serves as a substrate or binding partner in enzymatic assays to determine kinetic parameters and inhibition profiles.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify novel small-molecule modulators of the Rap1 interaction network for drug discovery.
• Academic & Institutional Research: A fundamental tool for universities and research institutes investigating yeast genetics, telomere biology, and eukaryotic cell cycle regulation.
• Protein Standard & Control: Acts as a purified standard for Western blotting, ELISA development, and other immunoassay techniques requiring a defined protein antigen.

Basic Information

Item	Detail
Product Name	Rap1-Interacting Protein, Yeast
CAS No.	146589-82-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Yeast Rap1-Interacting Protein; RPI1 Protein (Yeast); S. cerevisiae Rap1-Binding Protein; Rif1/2 Interacting Factor; Telomeric Silencing Protein Component; Recombinant Yeast RPI; Rap1 Pathway Protein; CAS 146589-82-4
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch of Rap1-Interacting Protein, Yeast is characterized for identity, purity, and functional activity. Certificates of Analysis (COA) detailing specifications such as purity by SDS-PAGE, endotoxin levels, and functional assay results are available upon request. Our quality management system supports research-grade and GMP-like manufacturing processes for critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product should be handled in a controlled environment to maintain activity and prevent contamination.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single major band at expected molecular weight
Purity (HPLC)	≥ 90%
Protein Content (UV)	As per COA
Biological Activity	Confirmed by binding assay (e.g., SPR, ELISA)
Endotoxin Level (LAL)	< 1.0 EU/µg
Buffer Composition	Specified in COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.