Description

Gelatinase B is a matrix metalloproteinase (MMP-9) enzyme critical for the degradation of extracellular matrix components, particularly type IV collagen and gelatin. This high-purity enzyme is essential for advanced biochemical research and industrial processes requiring precise proteolytic activity. It is primarily utilized by research institutions, diagnostic manufacturers, and biotechnology companies focused on areas such as cancer metastasis, tissue remodeling, and inflammatory disease studies.

Application

• Biomedical Research: Investigation of tumor cell invasion, angiogenesis, and tissue remodeling pathways in cancer and inflammatory diseases.
• Diagnostic Kit Development: A key component in the production of ELISA kits and other assays for detecting MMP-9 levels in clinical samples.
• Drug Discovery & Screening: Used as a target enzyme for high-throughput screening of novel MMP-9 inhibitors in pharmaceutical R&D.
• Cell Culture & Tissue Engineering: Employed to digest basement membrane matrices (e.g., Matrigel) for cell isolation and in 3D culture model preparation.
• Biochemical Analysis: Substrate for enzyme activity assays, kinetic studies, and zymography to assess proteolytic function.
• Industrial Biotechnology: Potential application in controlled protein hydrolysis processes where specific gelatin degradation is required.

Basic Information

Product Name	Gelatinase B
CAS No.	146480-36-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Matrix Metalloproteinase-9 (MMP-9); 92 kDa Gelatinase; 92 kDa Type IV Collagenase; Gelatinase B, Human Neutrophil; Type V Collagenase; CLG4B; GELB; MMP9; MANDP2
EINECS	Contact for details

Quality Control

Our Gelatinase B is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including activity assays, purity assessment, and specificity checks, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and absence of contaminating proteases is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into single-use vials to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (Activity)	Gelatinolytic activity confirmed by zymography or fluorogenic assay
Purity (SDS-PAGE)	> 90% (Single chain pro-form and active form)
Specific Activity	> 1,000 units/mg protein (Defined per batch COA)
Protein Content (Bradford)	Defined per batch COA
Endotoxin Level (LAL)	< 1.0 EU/µg (For research-grade material)
Solubility	Reconstitutes clearly in appropriate buffer (e.g., 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7.5)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.