Description

Mismatch Binding Protein CAS NO 146214-18-8 is a highly specialized recombinant protein engineered for the specific recognition and binding of DNA mismatches and other non-canonical DNA structures. This product is critical for advanced molecular biology research and diagnostic applications requiring high-fidelity DNA analysis. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies focused on genetic screening, mutation detection, and DNA repair studies.

Application

• Mutation Detection Assays: Core component in kits for identifying single nucleotide polymorphisms (SNPs), insertions, and deletions.
• DNA Repair Research: Used to study mismatch repair (MMR) pathways and associated proteins in vitro.
• Genetic Screening: Enables high-throughput screening for genetic variations in clinical and research settings.
• Biomarker Discovery: Facilitates the identification of DNA mismatch-related biomarkers for various diseases.
• Diagnostic Development: Integral to developing next-generation diagnostic tools for hereditary cancers and genetic disorders.
• Quality Control for Synthetic DNA: Used to verify the sequence fidelity of synthesized oligonucleotides and gene fragments.

Basic Information

Product Name	Mismatch Binding Protein
CAS No.	146214-18-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	MutS Protein (E. coli homolog); Mismatch Repair Protein; MMR Protein; DNA Mismatch-Binding Protein; hMutSα (human homolog component); MSH2-MSH6 Complex; GTBP; p160
EINECS	Contact for details

Quality Control

Our Mismatch Binding Protein is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing, including purity assessment by SDS-PAGE and HPLC, functional activity assays, and endotoxin level checks. A comprehensive Certificate of Analysis (COA) detailing identity, purity, activity, and stability is provided with every shipment to ensure traceability and compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single major band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Concentration	As per COA (typically 0.5 - 1.0 mg/mL)
Biological Activity (EMSA/Gel Shift)	Specific binding to mismatched DNA confirmed
Endotoxin Level (LAL)	< 1.0 EU/µg
Buffer Composition	Tris-HCl, Glycerol, DTT, NaCl (specifics on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.