Description

n-(Fluoresceinyl)-N'-(1-(6-(3-Mercapto-2-Benzyl-1-Oxopropyl)Amino)-1-Hexyl)Thiocarbamide is a specialized, high-value synthetic intermediate featuring a fluorescein core conjugated with a thiol-reactive maleimide derivative via a thiocarbamide linker. This compound matters for its dual functionality, combining the strong fluorescence properties of fluorescein with a reactive maleimide group designed for selective conjugation to sulfhydryl (-SH) groups under mild conditions. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, diagnostic assay development, and advanced material science, where it serves as a critical building block for creating fluorescently labeled probes, antibodies, and polymers.

Application

• Bioconjugation Reagent: For site-specific labeling of proteins, peptides, and antibodies via cysteine residues for fluorescence imaging (FLISA) and flow cytometry.
• Diagnostic Probe Synthesis: A key precursor in manufacturing fluorescent probes and tracers for in vitro diagnostics and biomedical research.
• Polymer Functionalization: Used to introduce fluorescent tags and reactive handles into polymers and hydrogels for sensor and biomaterial applications.
• Chemical Biology Tool: Enables the study of protein interactions, localization, and dynamics through fluorescence tagging.
• Surface Modification: For creating fluorescently labeled surfaces in microarray and biosensor chip technologies.

Basic Information

Product Name	n-(Fluoresceinyl)-N'-(1-(6-(3-Mercapto-2-Benzyl-1-Oxopropyl)Amino)-1-Hexyl)Thiocarbamide
CAS No.	145724-06-7
Molecular Formula	C37H33N3O6S2
Molecular Weight	679.80 g/mol
Synonyms	Fluorescein-5-maleimide (extended chain derivative); 5-((6-((3-Mercapto-2-benzyl-1-oxopropyl)amino)hexyl)thioureidyl)fluorescein; Fluorescein Maleimide Hexylamide Thioether; N-(Fluorescein-5-yl)-N'-[1-(6-[(3-mercapto-2-benzylpropanoyl)amino]hexyl)]thiourea; Fluorescein C6 Maleimide; Thiol-reactive Fluorescein derivative; Maleimidohexyl Fluorescein Thiocarbamide
EINECS	Contact for details

Quality Control

Our n-(Fluoresceinyl)-N'-(1-(6-(3-Mercapto-2-Benzyl-1-Oxopropyl)Amino)-1-Hexyl)Thiocarbamide is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research and diagnostic applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation of the thiol-reactive maleimide group and the thiocarbamide linker. For long-term stability, desiccate to protect against moisture. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC/UV)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (by UV-Vis)
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.