Description

Dabcyl-Gly-Leu-Arg-Thr-Gln-Ser-Phe-Ser-Edans is a synthetic, fluorogenic peptide substrate designed for protease activity assays. This compound is critical for high-sensitivity detection and quantification of enzymatic activity in biochemical research. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for studying protease kinetics, inhibitor screening, and assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the continuous, real-time monitoring of protease enzyme kinetics.
• Drug Discovery Screening: High-throughput screening (HTS) of potential protease inhibitor compounds in pharmaceutical R&D.
• Biochemical Research: Investigating the specific activity, substrate specificity, and catalytic mechanisms of various proteases.
• Diagnostic Kit Development: A key component in the development of sensitive in-vitro diagnostic (IVD) tests for clinical analysis.
• Quality Control in Biologics: Monitoring the presence and activity of proteases during the production and purification of biopharmaceuticals.
• Enzyme Characterization: Determining kinetic parameters such as Km and Vmax for detailed enzyme characterization studies.

Basic Information

Product Name	Dabcyl-Gly-Leu-Arg-Thr-Gln-Ser-Phe-Ser-Edans
CAS No.	145682-87-7
Molecular Formula	C68H98N20O21S2
Molecular Weight	1547.76 g/mol
Synonyms	Dabcyl-Gly-Leu-Arg-Thr-Gln-Ser-Phe-Ser-Edans; Dabcyl-GLRTSFS-Edans; Fluorogenic Protease Substrate; FRET-based Peptide Substrate; 5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid (Edans) and 4-((4-(Dimethylamino)phenyl)azo)benzoic acid (Dabcyl) labeled peptide; Thrombin Fluorogenic Substrate (related); Peptide-based FRET Probe; Caspase-3 Substrate Analog (related).
EINECS	Contact for details

Quality Control

Our Dabcyl-Gly-Leu-Arg-Thr-Gln-Ser-Phe-Ser-Edans is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and mass spectrometry for identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.