Description

Gln3 Protein CAS NO 145169-91-1 is a high-purity, biologically active protein essential for research in molecular biology and biochemistry. This product is critical for studies involving nitrogen metabolism and gene regulation in yeast and related organisms. It is primarily utilized by academic research institutions, biotechnology companies, and pharmaceutical R&D departments for protein-protein interaction studies, enzymatic assays, and as a standard in analytical workflows.

Application

• Biochemical Research: Used as a key reagent in studies of transcriptional regulation and nitrogen catabolite repression (NCR) pathways.
• Enzyme Substrate/Inhibitor Studies: Serves as a substrate or binding partner for investigating enzymatic activity and inhibition kinetics.
• Protein-Protein Interaction Assays: Essential for pull-down assays, co-immunoprecipitation (Co-IP), and yeast two-hybrid screening to map interaction networks.
• Diagnostic Development: Acts as a purified antigen for the development and validation of immunoassays and diagnostic kits.
• Structural Biology: Provides material for X-ray crystallography, NMR spectroscopy, and cryo-EM to determine 3D protein structures.
• Cell Signaling Research: Employed in cellular models to elucidate signaling pathways related to nutrient sensing and metabolic control.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify potential drug candidates that modulate its activity.
• Academic Teaching & Training: Serves as a standard protein in advanced laboratory courses for techniques in protein purification and characterization.

Basic Information

Product Name	Gln3 Protein
CAS No.	145169-91-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Gln3p; Nitrogen catabolite repression protein GLN3; GLN3 transcription factor; Yeast transcriptional activator GLN3; Regulatory protein GLN3; GLN3_YEAST; ORF YER040W
EINECS	Contact for details

Quality Control

Our Gln3 Protein is manufactured under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, functional activity assays, and endotoxin level checks. A comprehensive Certificate of Analysis (COA) detailing identity, purity, concentration, and biological activity is provided with every shipment to support your research compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Concentration	As per COA (typically 0.5-1.0 mg/mL)
Biological Activity	Confirmed by functional assay (e.g., DNA-binding EMSA)
Endotoxin Level	< 1.0 EU/μg
Buffer Composition	Specified on COA (e.g., Tris, NaCl, Glycerol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.