Description

n-Ethyl-n-(4-(2-(4-(1-Methylpyridino))Ethenyl)Phenyl)Glycine n-Hydroxysuccinimide Ester is a specialized, high-purity chemical reagent designed for advanced bioconjugation and labeling applications. Its core value lies in its reactive succinimidyl ester group, which enables efficient and stable covalent coupling to primary amines under mild aqueous conditions. This compound is essential for researchers and manufacturers in the life sciences sector, particularly for developing diagnostic assays, fluorescent probes, and antibody-drug conjugates (ADCs).

Application

• Bioconjugation Reagent: Primary application for covalently linking proteins, peptides, or antibodies to various labels, drugs, or solid supports via amine groups.
• Fluorescent Probe Synthesis: Used to prepare fluorescently tagged biomolecules for imaging, flow cytometry, and microscopy studies.
• Diagnostic Assay Development: Key component in the manufacture of enzyme-linked immunosorbent assays (ELISA) and other immunoassay detection systems.
• Antibody-Drug Conjugate (ADC) Payload Linkage: Serves as a critical linker-payload intermediate for attaching cytotoxic agents to monoclonal antibodies in targeted cancer therapies.
• Surface Functionalization: Employed to modify nanoparticles, microspheres, and biosensor surfaces with bioactive molecules.
• Protein Cross-linking & Immobilization: Facilitates the study of protein interactions or the creation of stable protein arrays on chips and plates.

Basic Information

Product Name	n-Ethyl-n-(4-(2-(4-(1-Methylpyridino))Ethenyl)Phenyl)Glycine n-Hydroxysuccinimide Ester
CAS No.	145128-12-7
Molecular Formula	C24H25N3O5
Molecular Weight	435.48 g/mol
Synonyms	NHS ester of N-Ethyl-N-[4-[2-(4-pyridin-1-ium-1-ylmethyl)ethenyl]phenyl]glycine; N-Ethyl-N-(4-(2-(4-(1-methylpyridin-1-ium-1-yl)vinyl)phenyl)glycine N-hydroxysuccinimide ester; N-Ethyl-N-(4-(2-(4-(N-methylpyridinio)vinyl)phenyl)glycine NHS ester; Bioconjugation linker NHS ester; Amine-reactive succinimidyl ester; Pyridinium-based NHS ester; N-Ethyl-N-(4-styrylphenyl)glycine NHS ester derivative
EINECS	Contact for details

Quality Control

Our n-Ethyl-n-(4-(2-(4-(1-Methylpyridino))Ethenyl)Phenyl)Glycine n-Hydroxysuccinimide Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.