Description

Cdc68 Protein CAS NO 145112-26-1 is a highly purified recombinant protein, also known as Pob3, which plays a critical role in chromatin organization and transcription regulation. This product is essential for researchers and developers in the life sciences sector, providing a reliable and consistent reagent for advanced biochemical studies. It is primarily utilized in academic research, pharmaceutical R&D, and biotechnology applications focused on chromatin structure, histone dynamics, and gene expression mechanisms.

Application

• Chromatin Remodeling Studies: Used as a key component in in vitro assays to investigate the mechanisms of chromatin assembly and disassembly.
• Transcription Research: Employed in biochemical studies to understand the role of the FACT complex in RNA polymerase II transcription elongation.
• Protein-Protein Interaction Analysis: Serves as a crucial bait or prey protein in pull-down assays, co-immunoprecipitation (Co-IP), and yeast two-hybrid screens.
• Enzymatic Activity Assays: Utilized in functional studies to assess the impact on histone chaperone activity and DNA replication processes.
• Drug Discovery Screening: Acts as a target protein in high-throughput screening platforms to identify potential therapeutic modulators of chromatin function.
• Biophysical Characterization: Used in techniques such as surface plasmon resonance (SPR) or isothermal titration calorimetry (ITC) to determine binding kinetics and affinity.

Basic Information

Product Name	Cdc68 Protein
CAS No.	145112-26-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pob3; Spt16/Cdc68; FACT complex subunit Pob3; Facilitates chromatin transcription complex subunit Pob3; Chromatin-specific transcription elongation factor 140 kDa subunit; Cdc68p; yFACT subunit Pob3
EINECS	Contact for details

Quality Control

Our Cdc68 Protein is produced under stringent conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes comprehensive analytical testing, including SDS-PAGE, Western Blot, and functional activity assays. A Certificate of Analysis (COA) detailing purity, concentration, endotoxin levels, and biological activity is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder or clear solution
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot)	Positive
Endotoxin Level	< 1.0 EU/µg
Protein Concentration	As per COA (typically 0.5-1.0 mg/mL)
Biological Activity	Confirmed by functional assay (e.g., chromatin remodeling or binding assay)
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.