Description

Cytochrome Bd-Ii Oxidase Subunit I, E Coli is a purified protein component of the cytochrome *bd*-II terminal oxidase complex derived from *Escherichia coli*. This enzyme subunit plays a critical role in bacterial respiration under microaerobic conditions, making it an essential reagent for studying bacterial bioenergetics and oxidative phosphorylation. It is primarily utilized by research scientists and biotechnologists in the fields of microbiology, enzymology, and structural biology for mechanistic studies, inhibitor screening, and as a component in reconstituted membrane systems.

Application

• Enzymatic Research & Mechanism Studies: Investigation of the catalytic cycle, proton pumping, and oxygen reduction mechanisms of the cytochrome *bd*-II oxidase complex.
• Antibacterial Drug Discovery: Target protein for screening and evaluating novel inhibitors targeting bacterial respiratory pathways, particularly for pathogens that rely on cytochrome *bd* oxidases.
• Bioenergetics & Membrane Protein Studies: Reconstitution into proteoliposomes to study electron transfer, proton motive force generation, and energy coupling in bacterial membranes.
• Structural Biology: Protein sample for X-ray crystallography, cryo-electron microscopy (cryo-EM), or NMR spectroscopy to determine high-resolution structures.
• Biochemical Assay Development: Key component in developing in vitro activity assays to measure oxidase function and inhibitor potency.
• Educational & Reference Standard: Used in advanced academic teaching and as a purified standard in comparative biochemical analyses.

Basic Information

Item	Detail
Product Name	Cytochrome Bd-Ii Oxidase Subunit I, E Coli
CAS No.	144997-58-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cytochrome bd-II oxidase subunit I; CydB protein, E. coli; Cytochrome bd-Ii ubiquinol oxidase subunit I; Terminal oxidase bd-II subunit I; EC 1.10.3.-; Respiratory chain complex IV (cytochrome bd type) subunit I
EINECS	Contact for details

Quality Control

Our Cytochrome Bd-Ii Oxidase Subunit I, E Coli is produced and handled under stringent conditions to ensure batch-to-batch consistency and biological activity. Each lot is characterized for purity and identity. Certificates of Analysis (COA) detailing specifications such as purity (by SDS-PAGE), concentration, and functional activity are provided and available upon request. Our quality system supports research requiring reliable, high-integrity biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in suitable aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot/ Mass Spec)	Conforms
Protein Concentration	As per COA
Biological Activity	Contact for details
Endotoxin Level	< 1.0 EU/µg (if applicable)
Buffer Composition	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.